Peptide nucleic acids as tools for single-molecule sequence detection and manipulation
| Autor: | Hagar Zohar, Carlos Bustamante, Craig L. Hetherington, Susan J. Muller |
|---|---|
| Rok vydání: | 2010 |
| Předmět: |
Peptide Nucleic Acids
Fluorophore Optical Tweezers Molecular Sequence Data Molecular Probe Techniques Bioengineering Peptide Article chemistry.chemical_compound Micromanipulation General Materials Science chemistry.chemical_classification Peptide nucleic acid Base Sequence Mechanical Engineering General Chemistry Sequence Analysis DNA Condensed Matter Physics chemistry Optical tweezers Biochemistry Molecular Probes Biophysics Nucleic acid Molecular probe DNA |
| Zdroj: | Nano letters. 10(11) |
| ISSN: | 1530-6992 |
| Popis: | The ability to strongly and sequence-specifically attach modifications such as fluorophores and haptens to individual double-stranded (ds) DNA molecules is critical to a variety of single-molecule experiments. We propose using modified peptide nucleic acids (PNAs) for this purpose and implement them in two model single-molecule experiments where individual DNA molecules are manipulated via microfluidic flow and optical tweezers, respectively. We demonstrate that PNAs are versatile and robust sequence-specific tethers. |
| Databáze: | OpenAIRE |
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