Development of Species-Specific Primers for Plasmodiophora brassicae, Clubroot Pathogen of Kimchi Cabbage
| Autor: | Jin Su Choi, Seul Gi Yang, Hong Gi Kim, Jeong Young Song |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
Plant Science
Plasmodiophora brassicae Biochemistry Microbiology Clubroot Botany medicine lcsh:Agriculture (General) Molecular Biology Pathogen biology Obligate fungi food and beverages Brassicaceae biology.organism_classification medicine.disease Quantitative real-time PCR lcsh:S1-972 Spore Detection genomic DNA Primer (molecular biology) Agronomy and Crop Science Clubroot pathogen Biotechnology |
| Zdroj: | Research in Plant Disease, Vol 20, Iss 1, Pp 21-24 (2014) |
| ISSN: | 1598-2262 |
| Popis: | Clubroot caused by the obligate biotrophic protist Plasmodiophora brassicae Woronin is one of the most damaging diseases of Brassicaceae family. In this study, we developed species-specific primer sets for rapid and accurate detection of P. brassicae. The primer sets developed amplified a specific fragment only from P. brassicae DNA while they did not amplify a band from 10 other soilborne pathogens or from Kimchi cabbage. In sensitivity test, the species-specific primer set ITS1-1/ITS1-2 could work for approximately 10 spores/ml of genomic DNA showing more sensitivity and accuracy than previous methods. With quantitative real-time PCR test, the primer set detected less spores of P. brassicae than before, confirming that the species-specific primer set could be useful for rapid and accurate detection of P. brassicae. |
| Databáze: | OpenAIRE |
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