Proteome and cytokine serum profiling to diagnose a mycosis fungoides
| Autor: | Anna A. Kubanova, Alexander I. Archakov, Ekaterina V. Brattseva, Maria A. Karpova, Sergei A. Moshkovskii, Mikhail A. Pyatnitskiy, Elizaveta E. Sokolova |
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| Rok vydání: | 2011 |
| Předmět: |
Adult
Male Chemokine Pathology medicine.medical_specialty Skin Neoplasms Proteome medicine.medical_treatment Clinical Biochemistry Protein Array Analysis Diagnosis Differential Mycosis Fungoides Blood serum Psoriasis Granulocyte Colony-Stimulating Factor medicine Humans CXCL10 Aged Skin Aged 80 and over Immunoassay Mycosis fungoides biology medicine.diagnostic_test Middle Aged medicine.disease Chemokine CXCL10 Interleukin 1 Receptor Antagonist Protein Cytokine Case-Control Studies Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Immunology biology.protein Female Interleukin-4 Antibody Biomarkers |
| Zdroj: | PROTEOMICS - Clinical Applications. 5:432-439 |
| ISSN: | 1862-8346 |
| DOI: | 10.1002/prca.201000165 |
| Popis: | Purpose: The aim of this study was to estimate a possibility of mycosis fungoides (MF) diagnostics based on protein profiling in blood serum. Experimental design: We obtained and analysed samples of blood serum from 23 patients with MF, and 29 psoriasis patients and 22 healthy donors as controls. Protein profiling was carried out using SELDI TOF MS SELDI-TOF and also profiling of 27 cytokines with multiplex immunoassay technology was implemented. Results: MS data analysis of sera did not give satisfactory statistical discrimination between the groups. Antibody-based cytokine profiling revealed a number of cytokines with a change in their concentrations in both MF and psoriasis (IL-1Ra, IL-4, G-CSF). The C-X-C motif chemokine 10 (IP-10, CXCL10) cytokine had a significantly increased concentration (p |
| Databáze: | OpenAIRE |
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