The Extent and Rate of the Appearance of the Major 110 and 30 kDa Proteolytic Fragments during Post-Mortem Aging of Beef Depend on the Glycolysing Rate of the Muscle and Aging Time: An LC-MS/MS Approach to Decipher Their Proteome and Associated Pathways
| Autor: | Declan J. Troy, Anne Maria Mullen, Mohammed Gagaoua |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Glycosylation Meat Proteome Proteolysis Muscle Proteins Proteomics 01 natural sciences Biological pathway Tandem Mass Spectrometry Apoptotic mitochondrial changes medicine Animals Muscle Skeletal Actin Chromatography High Pressure Liquid 2. Zero hunger medicine.diagnostic_test Chemistry 010401 analytical chemistry General Chemistry 0104 chemical sciences Fibrinogen complex Molecular Weight Biochemistry Postmortem Changes Cattle Electrophoresis Polyacrylamide Gel medicine.symptom General Agricultural and Biological Sciences 010606 plant biology & botany Muscle contraction |
| Zdroj: | Journal of agricultural and food chemistry. 69(1) |
| ISSN: | 1520-5118 |
| Popis: | Post-mortem (p-m) muscle undergoes a myriad of complex physical and biochemical changes prior to its conversion to meat, which are influential on proteolysis and hence tenderization. A more in-depth understanding of the mechanisms underpinning these dynamics is a key to consistently providing tender beef. Using an LC-MS/MS approach, with state-of-art mass spectrometry Q Exactive HF-X, the proteome and associated pathways contributing to the appearance of the proteolytic breakdown products appearing over 14 days p-m, at two important molecular weights (110 and 30 kDa) on 1D SDS-PAGE gels, have been investigated in beef longissimus thoracis et lumborum muscles exhibiting four rates of pH decline differentiated on the basis of time at pH 6 (fast glycolysing, or = medium > slow > very slow. The day 2 p-m appearance of the 30 kDa band was most evident for the fast glycolysing muscle with little or no evidence of appearance in slow and very slow. For days 7 and 14 p-m, the strength of appearance was dependent on glycolysing groups fast > medium > or = slow > very slow. LC-MS/MS analysis yielded a total of 22 unique proteins for the 110 kDa fragment and 13 for the 30 kDa, with 4 common proteins related to both the actin and fibrinogen complex. The Gene Ontology analysis revealed that a myriad of biological pathways are influential with many related to proteins involved primarily in muscle contraction and structure. Other pathways of interest include energy metabolism, apoptotic mitochondrial changes, calcium and ion transport, and so on. Interestingly, most of the proteins composing the fragments were so far identified as biomarkers of beef tenderness and other quality traits. |
| Databáze: | OpenAIRE |
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