An asymmetric junctional mechanoresponse coordinates mitotic rounding with epithelial integrity
| Autor: | Soichiro Yamada, Marjolein J. Vliem, Jooske L Monster, Joleen S Cheah, Martijn Gloerich, Johan de Rooij, Helen K. Matthews, Buzz Baum, Zaw Win, Lisa Donker |
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| Rok vydání: | 2021 |
| Předmět: |
Physiology
1.1 Normal biological development and functioning Mitosis Medical and Health Sciences Article Epithelium Cell Line Madin Darby Canine Kidney Cells 03 medical and health sciences 0302 clinical medicine Dogs Underpinning research medicine Animals 030304 developmental biology Epithelial barrier 0303 health sciences biology Cadherin Microfilament Proteins Epithelial Cells Cell Biology Adherens Junctions Vinculin Biological Sciences Cadherins Actins Cell biology Actin Cytoskeleton medicine.anatomical_structure Intercellular Junctions biology.protein Adhesion Mechanosensitive channels 030217 neurology & neurosurgery Cell Cycle and Division Developmental Biology |
| Zdroj: | The Journal of cell biology, vol 220, iss 5 The Journal of Cell Biology Journal of Cell Biology |
| ISSN: | 0021-9525 |
| Popis: | Monster, Donker, et al. demonstrate how epithelial cells can round up as they enter mitosis while still maintaining the integrity of the epithelial barrier. This requires an asymmetric composition of mitotic cell–cell junctions, which is established through an E-cadherin mechanoresponse in neighbors of mitotic cells. Epithelia are continuously self-renewed, but how epithelial integrity is maintained during the morphological changes that cells undergo in mitosis is not well understood. Here, we show that as epithelial cells round up when they enter mitosis, they exert tensile forces on neighboring cells. We find that mitotic cell–cell junctions withstand these tensile forces through the mechanosensitive recruitment of the actin-binding protein vinculin to cadherin-based adhesions. Surprisingly, vinculin that is recruited to mitotic junctions originates selectively from the neighbors of mitotic cells, resulting in an asymmetric composition of cadherin junctions. Inhibition of junctional vinculin recruitment in neighbors of mitotic cells results in junctional breakage and weakened epithelial barrier. Conversely, the absence of vinculin from the cadherin complex in mitotic cells is necessary to successfully undergo mitotic rounding. Our data thus identify an asymmetric mechanoresponse at cadherin adhesions during mitosis, which is essential to maintain epithelial integrity while at the same time enable the shape changes of mitotic cells. |
| Databáze: | OpenAIRE |
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