Anti-diabetic properties of genistein-chromium (III) complex in db/db diabetic mice and its sub-acute toxicity evaluation in normal mice
Autor: | Baosheng Zhao, Chaojun He, Ziyong Li, Pengshou Li, Ge Song, Qixiang Ma, Yujia Cao |
---|---|
Rok vydání: | 2020 |
Předmět: |
Blood Glucose
Chromium chemistry.chemical_element Genistein 010501 environmental sciences Pharmacology 01 natural sciences Biochemistry Mass Spectrometry Diabetes Mellitus Experimental Inorganic Chemistry 03 medical and health sciences chemistry.chemical_compound Mice 0302 clinical medicine In vivo Animals Hypoglycemic Agents Chelation 0105 earth and related environmental sciences Ethanol Glycogen Chemistry Insulin tolerance test Body Weight Glucose Tolerance Test Toxicity Molecular Medicine 030217 neurology & neurosurgery Chromatography Liquid |
Zdroj: | Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS). 62 |
ISSN: | 1878-3252 |
Popis: | Background In this study, chromium (III) complex was synthesized from genistein (GEN) which had good hypoglycemic activity and inorganic chromium (III) element, and its hypoglycemic activity and sub-acute toxicity were studied. Methods The genistein-chromium (III) complex was synthesized by chelating chromium with genistein in ethanol and its structure was determined by LC–MS, atomic absorption spectroscopy, UV–vis spectroscopy, infrared spectroscopy, elemental and thermodynamic analysis. The anti-diabetic activity of the complex was assessed in db/db mice and C57 mice by daily oral gavage for 4 weeks. The sub-acute toxicity test was carried out on KM mice with this complex. Results The molecular structure of this complex was inferred as a complex [CrGEN3] formed by three ligands and one chromium element. The complex could significantly improve the body weight of db/db mice, fasting blood glucose, random blood glucose, organ index, glycogen levels and the performance of OGTT (Oral Glucose Tolerance Test) and ITT (Insulin Tolerance Test) in db/db mice (p Conclusion The study of the genistein-chromium (III) complex showed that the complex had good hypoglycemic activity in vivo, and did not have the potential toxicity. These results would provide an important reference for the development of functional hypoglycemic foods or pharmaceuticals. |
Databáze: | OpenAIRE |
Externí odkaz: |