The role of amifostine in preventing radiotherapy induced testicular tissue damage in rats
Autor: | Ebru Karadag-Sari, Arzu Gezer |
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Rok vydání: | 2021 |
Předmět: |
Male
0301 basic medicine Germinal epithelium Histology Testicular tissue medicine.medical_treatment Radiation-Protective Agents Andrology 03 medical and health sciences Amifostine 0302 clinical medicine Testis In Situ Nick-End Labeling medicine Animals Rats Wistar Chemotherapy TUNEL assay 030102 biochemistry & molecular biology business.industry Significant difference General Medicine Rats Radiation therapy Medical Laboratory Technology Apoptosis 030220 oncology & carcinogenesis business medicine.drug |
Zdroj: | Biotechnic & Histochemistry. 97:215-221 |
ISSN: | 1473-7760 1052-0295 |
Popis: | The germinal epithelium of the adult testis is susceptible to radiation induced damage. Amifostine is a drug used to prevent the side effects of radiotherapy (RT) and chemotherapy. We investigated the protective role of amifostine against RT induced damage to rat testis using the TUNEL assay. We used adult male rats divided equally into four groups: untreated control group; amifostine group, 200 mg/kg amifostine/day for 3 days; RT-saline group, 2 Gy/day local irradiation of testes for 3 days; RT-amifostine group, 2 Gy/day local irradiation of testes for 3 days plus 200 mg/kg amifostine 30 min before each irradiation. Four weeks after treatment, rats were sacrificed for histological examination and apoptosis was assessed using the TUNEL method. The TUNEL staining density was obtained by evaluating separate seminiferous tubules selected randomly from each section using the stereological fractionator method. Apoptosis in the seminiferous tubules in the control group and amifostine groups were evaluated as spontaneous. Frequent apoptosis was observed in the RT-saline group; a statistically significant difference was observed between the RT treated and untreated groups. Administration of amifostine 30 min before RT protected the testicular germ cells against apoptosis. |
Databáze: | OpenAIRE |
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