Ciliopathy proteins establish a bipartite signaling compartment in a C. elegans thermosensory neuron
| Autor: | Michel R. Leroux, Willisa Liou, David H. Hall, Phuong Anh T. Nguyen |
|---|---|
| Rok vydání: | 2014 |
| Předmět: |
congenital
hereditary and neonatal diseases and abnormalities Sensory Receptor Cells Intermediate Filaments Models Biological Nephronophthisis medicine Animals Compartment (development) Cilia Caenorhabditis elegans Caenorhabditis elegans Proteins Cyclic GMP Tomography Molecular Biology Ion channel biology Cilium Temperature Ciliary transition zone Dendrites Cell Biology medicine.disease biology.organism_classification Lipids Actins Sensory neuron Cell Compartmentation Transport protein Cell biology Protein Transport Ciliopathy Intercellular Junctions medicine.anatomical_structure Guanylate Cyclase Mutation Signal transduction Signal Transduction Research Article Developmental Biology |
| Zdroj: | Journal of Cell Science. |
| ISSN: | 1477-9137 0021-9533 |
| DOI: | 10.1242/jcs.157610 |
| Popis: | How signaling domains form is an important, yet largely unexplored question. Here, we show that ciliary proteins help establish two contiguous, yet distinct cyclic GMP (cGMP) signaling compartments in Caenorhabditis elegans thermosensory AFD neurons. One compartment, a bona fide cilium, is delineated by proteins associated with Bardet–Biedl syndrome (BBS), Meckel syndrome and nephronophthisis at its base, and requires NPHP-2 (known as inversin in mammals) to anchor a cGMP-gated ion channel within the proximal ciliary region. The other, a subcompartment with profuse microvilli and a different lipid environment, is separated from the dendrite by a cellular junction and requires BBS-8 and DAF-25 (known as Ankmy2 in mammals) for correct localization of guanylyl cyclases needed for thermosensation. Consistent with a requirement for a membrane diffusion barrier at the subcompartment base, we reveal the unexpected presence of ciliary transition zone proteins where no canonical transition zone ultrastructure exists. We propose that differential compartmentalization of signal transduction components by ciliary proteins is important for the functions of ciliated sensory neurons. |
| Databáze: | OpenAIRE |
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