Effects of in vitro exposure to hay dust on the gene expression of chemokines and cell-surface receptors in primary bronchial epithelial cell cultures established from horses with chronic recurrent airway obstruction
| Autor: | Hollis N. Erb, Claudia L. Reyner, MaryBeth Matychak, Jean C Young, Dorothy M. Ainsworth |
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| Rok vydání: | 2009 |
| Předmět: |
Lipopolysaccharides
Male Chemokine Lipopolysaccharide Bronchi Receptors Cell Surface chemistry.chemical_compound Pulmonary Disease Chronic Obstructive Cell surface receptor Gene expression medicine Animals Horses Receptor Cells Cultured Recurrent airway obstruction General Veterinary biology Interleukin Dust Epithelial Cells General Medicine medicine.disease Animal Feed CXCL2 chemistry Gene Expression Regulation Immunology biology.protein Female Horse Diseases Chemokines |
| Zdroj: | American journal of veterinary research. 70(3) |
| ISSN: | 0002-9645 |
| Popis: | Objective—To examine effects of in vitro exposure to solutions of hay dust, lipopolysaccharide (LPS), or β-glucan on chemokine and cell-surface receptor (CSR) gene expression in primary bronchial epithelial cell cultures (BECCs) established from healthy horses and horses with recurrent airway obstruction (RAO). Sample Population—BECCs established from bronchial biopsy specimens of 6 RAO-affected horses and 6 healthy horses. Procedures—5-day-old BECCs were treated with PBS solution, hay dust solutions, LPS, or β-glucan for 6 or 24 hours. Gene expression of interleukin (IL)-8, chemokine (C-X-C motif) ligand 2 (CXCL2), IL-1β, toll-like receptor 2, toll-like receptor 4, IL-1 receptor 1, and glyceraldehyde 3–phosphate dehydrogenase was measured with a kinetic PCR assay. Results—Treatment with PBS solution for 6 or 24 hours was not associated with a significant difference in chemokine or CSR expression between BECCs from either group of horses. In all BECCs, treatment with hay dust or LPS for 6 hours increased IL-8, CXCL2, and IL-1β gene expression > 3-fold; at 24 hours, only IL-1β expression was upregulated by > 3-fold. In all BECCs, CSR gene expression was not increased following any treatment. With the exception of a 3.7-fold upregulation of CXCL2 in BECCs from RAO-affected horses (following 6-hour hay dust treatment), no differences in chemokine or CSR gene expression were detected between the 2 groups. At 24 hours, CXCL2 gene expression in all BECCs was downregulated. Conclusions and Clinical Relevance—Epithelial CXCL2 upregulation in response to hay dust particulates may incite early airway neutrophilia in horses with RAO. |
| Databáze: | OpenAIRE |
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