Development of an in vivo model to study clonal lineage relationships in hematopoietic cells using Brainbow2.1/Confetti mice
| Autor: | Jolanda. J. D. de Roo, Frank J. T. Staal, Edwin F. E. de Haas, Hans Vrolijk, Sandra A. Vloemans |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
Lineage (genetic)
Brainbow cassettes Confetti Biology retroviral-Cre expression hematopoiesis Cell biology Blood cell Haematopoiesis medicine.anatomical_structure lineage tracing cell tracking In vivo Lineage tracing medicine Cell tracking Stem cell multicolor flow cytometry Homeostasis Biotechnology Research Article |
| Zdroj: | Future Science OA |
| ISSN: | 2056-5623 |
| Popis: | Hematopoietic stem cells maintain the homeostasis of all blood cell progeny during development and repopulation-demanding events. To study the lineage relationships during hematopoiesis, increasingly complex cell tracing models are being developed. In this study, we describe adaptations to the original R26R-Confetti mouse model, which subsequently offers a relatively easy approach to study low complexity clonality during hematopoiesis, with special focus on B and T lymphocyte development. This protocol employs spatiotemporal Cre expression controlled by gammaretroviral transduction for efficient fluorescent protein cell marking. Transplantation of fluorescently marked Lin- cKit+ hematopoietic progenitor cells into Rag1-/- mice, resulted in the visualization of differentially contributing stem cell clones to various lineages. Our methodology is useful to study questions in fundamental and preclinical hematopoietic research and in vivo B- and T-cell development. Lay abstract Hematopoietic stem cells (HSCs) are responsible for the continuous production of specialized blood cells. B and T cells are blood cells that play an important role in the short and long-term immune system. These cells are frequently replenished, originating from one or multiple HSCs. Our methodology presents a relatively easy way to track the contribution of HSCs or progenitor cells to various mature blood cell types by the use of fluorescent protein cell marking. |
| Databáze: | OpenAIRE |
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