Nutrient Control of Yeast Gametogenesis Is Mediated by TORC1, PKA and Energy Availability
| Autor: | Hilla Weidberg, Angelika Amon, Fabien Moretto, Folkert J. van Werven, Gianpiero Spedale |
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| Přispěvatelé: | Koch Institute for Integrative Cancer Research at MIT, Weidberg, Hilla, Amon, Angelika B. |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Cell signaling Cancer Research Physiology Signal transduction Biochemistry Gametogenesis Fungal Reproduction Reproductive Physiology Transcription (biology) Medicine and Health Sciences Cell Cycle and Cell Division Promoter Regions Genetic Fungal Sporulation Genetics (clinical) biology Chromosome Biology Organic Compounds Messenger RNA Monosaccharides Nuclear Proteins Signaling cascades Spores Fungal PKA signaling cascade DNA-Binding Proteins Nucleic acids Meiosis Chemistry Cell Processes Physical Sciences Energy source Research Article Cell biology Saccharomyces cerevisiae Proteins lcsh:QH426-470 Saccharomyces cerevisiae Carbohydrates Repressor Mycology Protein Serine-Threonine Kinases Cell fate determination Glucose Signaling 03 medical and health sciences Genetics Protein kinase A Molecular Biology Transcription factor Ecology Evolution Behavior and Systematics Biology and life sciences Organic Chemistry Chemical Compounds biology.organism_classification Cyclic AMP-Dependent Protein Kinases Repressor Proteins lcsh:Genetics Glucose 030104 developmental biology RNA Transcription Factors |
| Zdroj: | PLOS PLoS Genetics, Vol 12, Iss 6, p e1006075 (2016) PLoS Genetics |
| Popis: | Cell fate choices are tightly controlled by the interplay between intrinsic and extrinsic signals, and gene regulatory networks. In Saccharomyces cerevisiae, the decision to enter into gametogenesis or sporulation is dictated by mating type and nutrient availability. These signals regulate the expression of the master regulator of gametogenesis, IME1. Here we describe how nutrients control IME1 expression. We find that protein kinase A (PKA) and target of rapamycin complex I (TORC1) signalling mediate nutrient regulation of IME1 expression. Inhibiting both pathways is sufficient to induce IME1 expression and complete sporulation in nutrient-rich conditions. Our ability to induce sporulation under nutrient rich conditions allowed us to show that respiration and fermentation are interchangeable energy sources for IME1 transcription. Furthermore, we find that TORC1 can both promote and inhibit gametogenesis. Down-regulation of TORC1 is required to activate IME1. However, complete inactivation of TORC1 inhibits IME1 induction, indicating that an intermediate level of TORC1 signalling is required for entry into sporulation. Finally, we show that the transcriptional repressor Tup1 binds and represses the IME1 promoter when nutrients are ample, but is released from the IME1 promoter when both PKA and TORC1 are inhibited. Collectively our data demonstrate that nutrient control of entry into sporulation is mediated by a combination of energy availability, TORC1 and PKA activities that converge on the IME1 promoter. Jane Coffin Childs Memorial Fund for Medical Research European Molecular Biology Organization (EMBO Long-term Fellowship) Weizmann Institute of Science (Israel National Postdoctoral Program for Advancing Women in Science) National Institutes of Health (U.S.) (NIH grant GM62207) Francis Crick Institute |
| Databáze: | OpenAIRE |
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