Trehalose liposomes induce apoptosis of breast tumor cells in vitro and in vivo
| Autor: | Keiji Kuwabara, Hideaki Ichihara, Yoko Matsumoto |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Biophysics Antineoplastic Agents Apoptosis Breast Neoplasms Biochemistry 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine In vivo Cell Line Tumor medicine Membrane fluidity Animals Humans skin and connective tissue diseases Molecular Biology Mice Inbred BALB C Liposome TUNEL assay Chemistry Cell Membrane Trehalose Cancer Cell Biology medicine.disease Molecular biology In vitro 030104 developmental biology 030220 oncology & carcinogenesis Liposomes Female Signal Transduction |
| Zdroj: | Biochemical and Biophysical Research Communications. 532:505-512 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2020.08.037 |
| Popis: | The inhibitory effects of trehalose liposomes (TL) comprising l -α-dimyristoylphosphatidylcholine (DMPC) and α-D-glucopyranosyl-α-D-glucopyranoside monomyritate (TreC14) were investigated on breast cancer MDA-MB-453 cells in vitro and in vivo. The IC50 values of TL for MDA-MB-453 cells were remarkably lower than those of DMPC liposomes. The inhibitory effects of TL on the proliferation of MDA-MB-453 cells mediated via apoptosis induction were observed following their accumulation on MDA-MB-453 cell membranes. The membrane fluidity of MDA-MB-453 cells increased after TL treatment, as evident from a fluorescence depolarization assay. TL induced the apoptosis of MDA-MB-453 cells through caspase activation and mitochondrial membrane potential reduction, and suppressed the nuclear factor kappa B activity. A remarkable reduction in tumor volume was observed in a human breast cancer mouse model topically treated with TL. Induction of apoptosis was evident in TL-treated breast cancer tumors of mice using the TUNEL assay. |
| Databáze: | OpenAIRE |
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