The spatial relationship between the musculature and the NADPH-diaphorase activity, 5-HT and FMRFamide immunoreactivities in redia, cercaria and adult Echinoparyphium aconiatum (Digenea)
| Autor: | S.N. Vodjanitskaja, H.-P. Fagerholm, Nadezhda B. Terenina, Elena A. Serbina, Margaretha K. S. Gustafsson, Oleg O. Tolstenkov |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Central Nervous System
Nervous system Serotonin Pathology medicine.medical_specialty Phalloidin Confocal Immunocytochemistry Biology chemistry.chemical_compound Nitrergic Neurons medicine Animals FMRFamide Microscopy Confocal NADPH Dehydrogenase Cell Biology General Medicine Anatomy Immunohistochemistry Staining medicine.anatomical_structure chemistry Larva Peripheral nervous system Microscopy Electron Scanning Trematoda Developmental Biology |
| Zdroj: | Tissue and Cell. 38:151-157 |
| ISSN: | 0040-8166 |
| DOI: | 10.1016/j.tice.2006.01.003 |
| Popis: | The spatial relationship between the musculature and the NADPH-diaphorase (NADPH-d) activity, 5-HT and FMRFamide immunoreactivities in redia, cercaria and adult Echinoparyphium aconiatum was studied using scanning electron microscopy (SEM), NADPH-d histochemistry, immunocytochemistry, and confocal scanning laser microscopy (CSLM). TRITC-conjugated phalloidin was used to stain the musculature. Staining for NADPH-d was observed in the central (CNS) and peripheral nervous system (PNS) of all three stages. NADPH-d positive nerves occurred very close to muscle fibres. 5-HT-immunoreactive (5-HT-IR) nerve cells and fibres occurred in the CNS and PNS and close to muscle fibres. FMRFamide-IR nerve fibres were observed in the CNS and PNS of adult worms. This is the first time, the presence of the NADPH-d has been demonstrated in the larval as well as the adult stages of a fluke. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect