Cdc2‐like kinase 2 (Clk2) promotes early neural development inXenopusembryos
| Autor: | Hitoshi Yoshida, Atsushi Suzuki, Yuta Yoshimoto, Kimiko Takebayashi-Suzuki, Regina Putri Virgirinia, Makoto Nakamura, Maya Okada, Naoto Ueno, Hajime Akao, Nusrat Jahan, Fatchiyah Fatchiyah |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Embryo
Nonmammalian animal structures Xenopus Ectoderm Xenopus Proteins Biology Bone morphogenetic protein Fibroblast growth factor Nervous System Xenopus laevis 03 medical and health sciences 0302 clinical medicine medicine Animals 030304 developmental biology Mitogen-Activated Protein Kinase Kinases Neural Plate 0303 health sciences Embryogenesis Gene Expression Regulation Developmental Cell Differentiation Cyclin-Dependent Kinase 5 Cell Biology biology.organism_classification Cell biology Fibroblast Growth Factors medicine.anatomical_structure Bone Morphogenetic Proteins embryonic structures Neural development Neural plate 030217 neurology & neurosurgery Signal Transduction Developmental Biology Morphogen |
| Zdroj: | Development, Growth & Differentiation. 61:365-377 |
| ISSN: | 1440-169X 0012-1592 |
| DOI: | 10.1111/dgd.12619 |
| Popis: | Neural induction and patterning in vertebrates are regulated during early development by several morphogens, such as bone morphogenetic proteins (BMPs) and fibroblast growth factors (FGFs). Ventral ectoderm differentiates into epidermis in response to BMPs, whereas BMP signaling is tightly inhibited in the dorsal ectoderm which develops into neural tissues. Here, we show that Cdc2-like kinase 2 (Clk2) promotes early neural development and inhibits epidermis differentiation in Xenopus embryos. clk2 is specifically expressed in neural tissues along the anterior-posterior axis during early Xenopus embryogenesis. When overexpressed in ectodermal explants, Clk2 induces the expression of both anterior and posterior neural marker genes. In agreement with this observation, overexpression of Clk2 in whole embryos expands the neural plate at the expense of epidermal ectoderm. Interestingly, the neural-inducing activity of Clk2 is increased following BMP inhibition and activation of the FGF signaling pathway in ectodermal explants. Clk2 also downregulates the level of p-Smad1/5/8 in cooperation with BMP inhibition, in addition to increasing the level of activated MAPK together with FGF. These results suggest that Clk2 plays a role in early neural development of Xenopus possibly via modulation of morphogen signals such as the BMP and FGF pathways. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
| Nepřihlášeným uživatelům se plný text nezobrazuje | K zobrazení výsledku je třeba se přihlásit. |