Photodynamic Action of Proflavine on Coliphage T3 I. Kinetics of Inactivation
| Autor: | Dean Fraser, Heman Witmer |
|---|---|
| Rok vydání: | 1971 |
| Předmět: |
Time Factors
Light Statistics as Topic Immunology Kinetics Coliphages Microbiology chemistry.chemical_compound Virology Spectrophotometry Escherichia coli medicine Coliphage Proflavine Initial rate biology medicine.diagnostic_test Temperature Drug Resistance Microbial Darkness biology.organism_classification Culture Media chemistry Biochemistry Insect Science Bacterial Viruses Biophysics Acridines Saturation vapor curve Saturation (chemistry) |
| Zdroj: | Journal of Virology. 7:314-318 |
| ISSN: | 1098-5514 0022-538X |
| DOI: | 10.1128/jvi.7.3.314-318.1971 |
| Popis: | The photodynamic inactivation of coliphage T3 was studied over a wide range of concentrations of the dye proflavine. With 2 × 10 7 phage/ml, two modes of inactivation were observed. Between 0.25 and 12 to 13 μg/ml, inactivation was biphasic. There was an initial first-order inactivation (R×1) which became temporally associated with an apparently multiorder process (R×2) at higher light doses. Dye concentrations above 12 to 13 μg/ml showed only two-target inactivation curves (R×3), except at high dye concentrations where processes kinetically identical to R×1 and R×2 reappeared. R×2 showed a normal rectangular hyperbolic saturation curve but R×1 and R×3 appeared to saturate prematurely. The saturation behavior of R×1 and R×2 was independent of phage concentration, but R×3 was lost at phage titers above 2 × 10 7 /ml. No dark inactivation was seen with R×1 and R×2 subsequent to a period of illumination. With R×3, an exponential dark inactivation was seen for at least 1 hr after a period of illumination. The dye-phage system equilibrated immediately, at any temperature, at proflavine concentrations where R×1 and R×2 occurred. With R×3, prolonged equilibration times were necessary. Moreover, there was a temperature effect. The rate of inactivation at equilibrium was temperature-dependent, whereas the initial rate at which equilibrium was approached was essentially temperature-independent. |
| Databáze: | OpenAIRE |
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