A continuous spectrophotometric assay for sucrose phosphate synthetase
| Autor: | Erwin Latzko, David A. Walker, Simon P. Robinson, Ian E. Woodrow, S. Harbron, G.J. Kelly |
|---|---|
| Rok vydání: | 1980 |
| Předmět: |
chemistry.chemical_classification
Sucrose biology Kinase Fructosephosphates Biophysics Cell Biology Plants biology.organism_classification Phosphate Zea mays Biochemistry chemistry.chemical_compound Enzyme chemistry Glucosyltransferases Lactate dehydrogenase Spinach Spectrophotometry Ultraviolet Plants Edible Molecular Biology Triticum Pyruvate kinase |
| Zdroj: | Analytical Biochemistry. 107:56-59 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(80)90491-1 |
| Popis: | A continuous spectrophotometric assay for sucrose phosphate synthetase is described. In this assay, the production of UDP is coupled to NADH oxidation by the enzymes nucleoside-5′-diphosphate kinase, pyruvate kinase, and lactate dehydrogenase. The assay could not be used with crude extracts, but was found suitable for use with partially purified sucrose phosphate synthetase from the leaves of spinach, wheat, and maize. It has obvious advantages for kinetic studies. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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