The influence of inhibition of acid phosphatase, β-N-acetylglucosaminidase and lactate dehydrogenase present in the sperm of ide (Leuciscus idus) on the percentage of fertilised eggs
| Autor: | Daniel Żarski, Beata Sarosiek, Katarzyna Palińska-Żarska, Katarzyna Dryl |
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| Rok vydání: | 2018 |
| Předmět: |
Male
0301 basic medicine Zygote Cyprinidae Andrology 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Endocrinology Human fertilization Food Animals Semen Lactate dehydrogenase Acetamides Acetylglucosaminidase Animals Enzyme Inhibitors Sperm motility Fertilisation Molybdenum chemistry.chemical_classification 030219 obstetrics & reproductive medicine L-Lactate Dehydrogenase biology Gossypol Acid phosphatase General Medicine Spermatozoa Sperm Fertility 030104 developmental biology Enzyme chemistry biology.protein Animal Science and Zoology |
| Zdroj: | Animal Reproduction Science. 195:96-101 |
| ISSN: | 0378-4320 |
| Popis: | This study investigated how the inhibition of certain enzymes present in ide sperm influences sperm motility and the percentage of fertilised eggs. The enzymes studied were acid phosphatase (AcP), β-N-acetylglucosaminidase (β-NAGase) and lactate dehydrogenase (LDH). None of the inhibitors affected ide sperm motility parameters. The addition of gossypol (a LDH inhibitor) caused a considerable increase in the percentage of fertilised eggs (92–95% compared to 63% in the control). The inhibition of AcP caused a considerable decrease in fertility rate – at the highest inhibitor dose, the percentage of fertilised eggs decreased to 26%. A similar effect was seen after the addition of acetamide (a β-NAGase inhibitor), but in this case the highest dose caused complete inhibition of fertilisation. The results presented here indicate the importance of AcP and β-NAGase in the process of ide fertilisation. |
| Databáze: | OpenAIRE |
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