Imaging of Lymph Node Micrometastases Using an Oncolytic Herpes Virus and [18F]FEAU PET
| Autor: | Hedvig Hricak, Jatin P. Shah, Sheng Gang, Peter Brader, Ronald G. Blasberg, Yuman Fong, Ziv Gil, Kaitlyn J. Kelly, Richard J. Wong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Pathology
medicine.medical_specialty Radiology and Medical Imaging Sentinel lymph node Radiology and Medical Imaging/Ultrasonography Melanoma Experimental Oncology/Skin Cancers Surgery/Head and Neck Surgery Oncolytic herpes virus 03 medical and health sciences Mice 0302 clinical medicine Biopsy medicine Animals Surgery/Surgical Oncology Radiology and Medical Imaging/Computer Tomography Lymph node Herpesviridae 030304 developmental biology Oncology/Prostate Cancer 0303 health sciences Multidisciplinary Oncology/Head and Neck Cancers medicine.diagnostic_test business.industry Melanoma Arabinofuranosyluracil Radiology and Medical Imaging/Magnetic Resonance Imaging medicine.disease 3. Good health Oncolytic virus Oncolytic Viruses medicine.anatomical_structure Oncology Positron emission tomography 030220 oncology & carcinogenesis Lymphatic Metastasis Positron-Emission Tomography Radiology and Medical Imaging/PET and SPECT Imaging Surgery Lymph Lymph Nodes Radiology and Medical Imaging/Radionuclide Imaging Surgery/Pediatric Surgery business Research Article |
| Zdroj: | PLoS ONE |
| ISSN: | 1932-6203 |
| Popis: | Background In patients with melanoma, knowledge of regional lymph node status provides important information on outlook. Since lymph node status can influence treatment, surgery for sentinel lymph node (SLN) biopsy became a standard staging procedure for these patients. Current imaging modalities have a limited sensitivity for detection of micrometastases in lymph nodes and, therefore, there is a need for a better technique that can accurately identify occult SLN metastases. Methodology/Principal Findings B16-F10 murine melanoma cells were infected with replication-competent herpes simplex virus (HSV) NV1023. The presence of tumor-targeting and reporter-expressing virus was assessed by [18F]-2′-fluoro-2′-deoxy-1-β-D-β-arabinofuranosyl-5-ethyluracil ([18F]FEAU) positron emission tomography (PET) and confirmed by histochemical assays. An animal foot pad model of melanoma lymph node metastasis was established. Mice received intratumoral injections of NV1023, and 48 hours later were imaged after i.v. injection of [18F]FEAU. NV1023 successfully infected and provided high levels of lacZ transgene expression in melanoma cells. Intratumoral injection of NV1023 resulted in viral trafficking to melanoma cells that had metastasized to popliteal and inguinal lymph nodes. Presence of virus-infected tumor cells was successfully imaged with [18F]FEAU-PET, that identified 8 out of 8 tumor-positive nodes. There was no overlap between radioactivity levels (lymph node to surrounding tissue ratio) of tumor-positive and tumor-negative lymph nodes. Conclusion/Significance A new approach for imaging SLN metastases using NV1023 and [18F]FEAU-PET was successful in a murine model. Similar studies could be translated to the clinic and improve the staging and management of patients with melanoma. |
| Databáze: | OpenAIRE |
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