The secretion of plasminogen activators by human myeloid leukemic cells in vitro
| Autor: | Elaine Lynette Wilson, Peter Jacobs, Eugene B. Dowdle |
|---|---|
| Rok vydání: | 1983 |
| Předmět: |
Urokinase
Myeloid Immunology Myeloid leukemia Combination chemotherapy Cell Biology Hematology Biology Urokinase-Type Plasminogen Activator Biochemistry Molecular biology Tissue plasminogen activator In vitro Enzyme Activation Leukemia Myeloid Acute Plasminogen Activators medicine.anatomical_structure Leukemia Myeloid medicine Humans Peripheral blood cell Plasminogen activator Cells Cultured Granulocytes medicine.drug |
| Zdroj: | Blood. 61:568-574 |
| ISSN: | 1528-0020 0006-4971 |
| Popis: | Peripheral blood cell preparation from 23 normal subjects and 72 patients with acute and 32 patients with chronic myeloid leukemia were cultured in vitro and released plasminogen activators were analyzed. The quantity of plasminogen activator secreted by leukemic cells varied widely and could not be correlated with the clinical severity of the disease. Immunochemical and electrophoretic techniques have been used to show that normal peripheral blood granulocytes released exclusively urokinase-like plasminogen activator, whereas leukemic cells secreted either urokinase or a tissue activator-like enzyme. The molecular species of enzyme released by acute myeloid leukemic cells may serve as a diagnostic marker of relevance to the management of this disease, since patients with acute myeloid leukemia whose cells released only tissue plasminogen activator did not respond to combination chemotherapy. Tissue plasminogen activators released by leukemic cells may display an unusual electrophoretic pattern that resembles that shown by urokinase. Immunochemical procedures are therefore essential for the correct identification of these enzymes. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|