Upregulated Local Cytokine Production in Recurrent Tonsillitis Compared with Tonsillar Hypertrophy
Autor: | Karin Ågren, Ulf Andersson, Ingemar Ernberg, Jan Andersson, Annika Linde, Carl-Erik Nord, Britt Nordlander |
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Rok vydání: | 1995 |
Předmět: |
Male
Pathology medicine.medical_specialty medicine.medical_treatment Palatine Tonsil Muscle hypertrophy Interferon-gamma stomatognathic system Antigen Recurrence medicine Humans Child Lymphotoxin-alpha Cells Cultured Tumor Necrosis Factor-alpha business.industry Interleukins Infant Hypertrophy General Medicine Tonsillectomy Tonsillitis Cytokine medicine.anatomical_structure Otorhinolaryngology Cell culture Child Preschool Tonsil Immunology Cytokines Immunohistochemistry Female Tumor necrosis factor alpha business |
Zdroj: | Acta Oto-Laryngologica. 115:689-696 |
ISSN: | 1651-2251 0001-6489 |
Popis: | In children with recurrent tonsillitis there may be persistent antigen deposition in tonsil tissue. even between exacerbations. If so, upregulation of immunocompetent cells should occur continuously, in contrast to tonsil tissue from children with tonsillar hypertrophy. The cytokine pattern was studied in cell suspensions prepared from tonsils obtained from 12 children undergoing tonsillectomy. The study group comprised 6 children with recurrent tonsillitis and 6 who had a history of tonsillar hypertrophy causing sleep apnea. Cytokine-producing cells (IL-1alpha, IL-1beta, TNFalpha, IL-6, IL-8, IL-2, IFNgamma, TNFbeta, IL-10 and IL-4) were characterized at the single-cell level by use of cytokine-specific monoclonal antibodies and indirect immunofluorescence technique. A constitutive production of IL-1alpha, IL-1beta, TNFalpha, and IL-8 was found in both groups (10-300/10(5) cells). However, the frequency of spontaneous IL-2, IFNgamma, TNFalpha, IL-6 and IL-10 was consistently low (10 +/- 10 cells) in both groups. Following restimulation by T-cell receptor ligation, using immobilized anti-CD3 mAb, with concentrations chosen so that it did not activate resting cells, increased frequencies of TNFalpha, IL-6, IL-8, IL-2, IFNgamma, IL-4 and 1L-10 synthesizing cells were induced in the recurrent tonsillitis group. Significantly higher incidences of IL-1beta, IL-6 and IL-2 producing cells were found in the recurrent tonsillitis group (60-200/10(5) cells, p |
Databáze: | OpenAIRE |
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