A multidrug-resistant Stenotrophomonas maltophilia clinical isolate from Kamuzu Central Hospital, Malawi
| Autor: | Abel Phiri, Geoffrey Peterkins Kumwenda, Burnet Lichapa, Watipaso Kasambara, Faheema E. Choonara, Kenneth Chizani, Alick Banda |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Kamuzu Central Hospital
Malawi Carbapenem medicine.drug_class Stenotrophomonas maltophilia Antibiotics Microbial Sensitivity Tests Fosfomycin Meropenem carbapenem Microbiology Drug Resistance Multiple Bacterial medicine Humans Arbekacin Original Research biology General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification Hospitals Anti-Bacterial Agents ceftriaxone Multiple drug resistance Colistin Gram-Negative Bacterial Infections medicine.drug |
| Zdroj: | Malawi Medical Journal |
| ISSN: | 1995-7262 |
| Popis: | BackgroundStenotrophomonas maltophilia is a significant opportunistic pathogen that is associated with high mortality in immunocompromised individuals. In this study, we describe a multidrug-resistant (MDR) S. maltophilia clinical isolate from Kamuzu Central Hospital (KCH), Lilongwe, Malawi.MethodsA ceftriaxone and meropenem nonsusceptible isolate (Sm-MW08), recovered in December 2017 at KCH, was referred to the National Microbiology Reference Laboratory for identification. In April 2018, we identified the isolate using MALDI Biotyper mass spectrometry and determined its antimicrobial susceptibility profile using microdilution methods. Sm-MW08 was analysed by S1- PFGE, PCR, and Sanger sequencing, in order to ascertain the genotypes that were responsible for the isolate`s multidrug-resistance (MDR) phenotype.ResultsSm-MW08 was identified as S. maltophilia and exhibited resistance to a range of antibiotics, including all β-lactams, aminoglycosides (except arbekacin), chloramphenicol, minocycline, fosfomycin and fluoroquinolones, but remained susceptible to colistin and trimethoprim-sulfamethoxazole. The isolate did not harbour any plasmid but did carry chromosomally-encoded blaL1 metallo-βlactamase and blaL2 β-lactamase genes; this was consistent with the isolate’s resistance profile. No other resistance determinants were detected, suggesting that the MDR phenotype exhibited by Sm-MW08 was innate.ConclusionHerein, we have described an MDR S. maltophilia from KCH in Malawi, that was resistant to almost all locally available antibiotics. We therefore recommend the practice of effective infection prevention measures to curtail spread of this organism. |
| Databáze: | OpenAIRE |
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