Identification of specific biomarkers for gastric adenocarcinoma by ITRAQ proteomic approach
| Autor: | Shengli Xue, Jin Zhou, Jenny Lee Song, Zaixiang Tang, Qiaoming Zhi, Xiaoxiao Wang, Congcong Shen, Yao-Hua Song, Yangxin Li, Songbai Liu, Weichang Chen, Chaofan Wu, Meiyu Bao |
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| Rok vydání: | 2016 |
| Předmět: |
Male
Proteomics 0301 basic medicine Blotting Western Motility Adenocarcinoma Article 03 medical and health sciences 0302 clinical medicine Western blot Cell Movement Stomach Neoplasms Tandem Mass Spectrometry Biomarkers Tumor medicine Humans Neoplasm Invasiveness Aged Regulation of gene expression Gene knockdown Multidisciplinary medicine.diagnostic_test Chemistry Cell migration Middle Aged medicine.disease Molecular biology Neoplasm Proteins Gene Expression Regulation Neoplastic Blot Gene Ontology 030104 developmental biology Fluorescent Antibody Technique Direct 030220 oncology & carcinogenesis Female Energy Metabolism |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | The aim of this study was to identify biomarkers for gastric cancer (GC) by iTRAQ. Using proteins extracted from a panel of 4 pairs of gastric adenocarcinoma samples (stage III-IV, Her-2 negative), we identified 10 up regulated and 9 down regulated proteins in all four pairs of GC samples compared to adjacent normal gastric tissue. The up regulated proteins are mainly involved in cell motility, while the down regulated proteins are mitochondrial enzymes involved in energy metabolism. The expression of three up regulated proteins (ANXA1, NNMT, fibulin-5) and one of the down regulated proteins (UQCRC1) was validated by Western Blot in 97 GC samples. ANXA1 was up regulated in 61.36% of stage I/II GC samples compared to matched adjacent normal gastric tissue, and its expression increased further in stage III/IV samples. Knockdown of ANXA1 by siRNA significantly inhibited GC cell migration and invasion, whereas over expression of ANXA1 promoted migration and invasion. We found decreased expression of UQCRC1 in all stages of GC samples. Our data suggest that increased cell motility and decreased mitochondrial energy metabolism are important hallmarks during the development of GC. |
| Databáze: | OpenAIRE |
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