Skin application of the nonsteroidal anti-inflammatory drug ketoprofen downmodulates the antigen-presenting ability of Langerhans cells in mice
| Autor: | Katsuhiko Akiyama, Kenji Kabashima, Kenji Atarashi, Yoshiki Tokura |
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| Rok vydání: | 2008 |
| Předmět: |
Ketoprofen
Langerhans cell Picryl Chloride Dermatology Administration Cutaneous Dermatitis Contact Immunofluorescence Dinoprostone Picryl chloride Mice chemistry.chemical_compound Antigen In vivo medicine Animals Cells Cultured Skin CD86 Antigen Presentation Mice Inbred BALB C integumentary system medicine.diagnostic_test Anti-Inflammatory Agents Non-Steroidal Histocompatibility Antigens Class II Molecular biology In vitro medicine.anatomical_structure Microscopy Fluorescence chemistry Langerhans Cells Immunology Female B7-2 Antigen medicine.drug |
| Zdroj: | British Journal of Dermatology. 159:306-313 |
| ISSN: | 1365-2133 0007-0963 |
| Popis: | Summary Background Ketoprofen (KP) is widely used as a topical nonsteroidal anti-inflammatory drug that inhibits prostaglandin (PG) biosynthesis. As PGE2 upregulates the antigen-presenting activity of Langerhans cells (LCs), i.e. migration to lymph nodes and expression of immunocompetent molecules, modulation of LC functions resulting from topical application of KP is an issue to be clarified. Objectives To investigate the in vivo effect of KP application to the skin and the in vitro effect of KP addition to the culture on the antigen-presenting ability of murine LCs. Methods Ears of BALB/c mice were painted with picryl chloride (PCl) hapten, KP or both. An immunofluorescence study of epidermal sheets and a flow cytometric analysis of epidermal cell suspensions from the treated ears were performed. Results PCl altered the morphology of LCs and reduced their number, and simultaneous application of 10% KP maintained LC morphology and number. KP at 5% or 10% clearly decreased the PCl-augmented expression of major histocompatibility complex class II and CD86 on LCs. In cultivation of freshly isolated epidermal cells, 5 mmol L−1 KP inhibited the culture-promoted expression of these molecules on LCs, whereas 100 μmol L−1 indomethacin was not inhibitory. The further addition of PGE2 to the KP-containing epidermal cell culture did not restore the expression of these molecules. Moreover, topical application of 10% KP to the sensitizing sites suppressed the development of contact hypersensitivity to PCl. Conclusions KP may have the potential to inhibit the antigen-presenting ability of LCs, in a PGE2-independent manner. |
| Databáze: | OpenAIRE |
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