Population and Single-Cell Analysis of Human Cardiogenesis Reveals Unique LGR5 Ventricular Progenitors in Embryonic Outflow Tract
| Autor: | Chikai Zhou, Makoto Sahara, Jesper Sohlmer, Chuen Yan Leung, Kristine Bylund, Peter J. Gruber, Kenneth R. Chien, Nevin Witman, Federica Santoro, Mimmi M. Mononen |
|---|---|
| Jazyk: | angličtina |
| Předmět: |
TNNT2
Heart Ventricles Organogenesis Population Human Embryonic Stem Cells LIM-Homeodomain Proteins Biology General Biochemistry Genetics and Molecular Biology Cell Line Receptors G-Protein-Coupled 03 medical and health sciences 0302 clinical medicine Animals Humans Myocytes Cardiac Progenitor cell education Induced pluripotent stem cell Molecular Biology Cells Cultured Embryonic Stem Cells 030304 developmental biology 0303 health sciences education.field_of_study Multipotent Stem Cells Myocardium Wnt signaling pathway LGR5 Endothelial Cells Cell Differentiation Cell Biology Embryonic stem cell Cell biology Mice Inbred C57BL cardiovascular system ISL1 Single-Cell Analysis 030217 neurology & neurosurgery Developmental Biology |
| Zdroj: | Developmental Cell |
| ISSN: | 1534-5807 |
| DOI: | 10.1016/j.devcel.2019.01.005 |
| Popis: | Summary The morphogenetic process of mammalian cardiac development is complex and highly regulated spatiotemporally by multipotent cardiac stem/progenitor cells (CPCs). Mouse studies have been informative for understanding mammalian cardiogenesis; however, similar insights have been poorly established in humans. Here, we report comprehensive gene expression profiles of human cardiac derivatives from multipotent CPCs to intermediates and mature cardiac cells by population and single-cell RNA-seq using human embryonic stem cell-derived and embryonic/fetal heart-derived cardiac cells micro-dissected from specific heart compartments. Importantly, we discover a uniquely human subset of cono-ventricular region-specific CPCs, marked by LGR5. At 4 to 5 weeks of fetal age, the LGR5+ population appears to emerge specifically in the proximal outflow tract of human embryonic hearts and thereafter promotes cardiac development and alignment through expansion of the ISL1+TNNT2+ intermediates. The current study contributes to a deeper understanding of human cardiogenesis, which may uncover the putative origins of certain human congenital cardiac malformations. |
| Databáze: | OpenAIRE |
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