Immunogenicity of Various Beta-Lactam Antibiotic-Protein Conjugates and Cross-Reactivity of the Antibodies Produced in Guinea Pig
| Autor: | Hiroshi Shionoya, Naruo Katsutani |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
medicine.medical_treatment
Guinea Pigs Immunology Serum albumin Enzyme-Linked Immunosorbent Assay Cross Reactions medicine.disease_cause Binding Competitive Cross-reactivity medicine Animals Immunology and Allergy Bovine serum albumin Antiserum biology Chemistry Immunogenicity Beta lactam antibiotic Serum Albumin Bovine General Medicine Anti-Bacterial Agents Cephalosporins Antibody Formation biology.protein Female Antibody Adjuvant |
| Zdroj: | International Archives of Allergy and Immunology. 100:128-134 |
| ISSN: | 1423-0097 1018-2438 |
| DOI: | 10.1159/000236399 |
| Popis: | Immunogenic properties of bovine serum albumin (BSA) conjugates of various beta-lactam antibiotics (ABs) and cross-reactivity of the produced antibodies were investigated in guinea pigs. Each AB-BSA conjugate was immunized with complete Freund's adjuvant. Following the second injection, AB-specific antibodies were detected with high titers. Produced antibodies showed concentration-dependent inhibition by the corresponding AB. Cross-reactivity between the produced antibodies and ABs was evaluated with two methods, i.e. by testing for reactivity with the various AB-ovalbumin conjugates, and by assaying for inhibition of enzyme-linked immunosorbent assay (ELISA) by the various ABs. The latter method could detect minor cross-reactions more sensitively than the former one. The results suggested that similarity of the acyl side chain of a cephalosporin nucleus or a penicillin nucleus contributes to immunological cross-reactivity. |
| Databáze: | OpenAIRE |
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