Effects of stimulated aggrecanolysis on nanoscale morphological and mechanical properties of wild-type and aggrecanase-resistant mutant mice cartilages
| Autor: | Peter Vee Sin Lee, Md. Hemayet Uddin, Huabin Wang, Xuehua Zhang, Fraser M. Rogerson |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Cartilage Articular Proteolysis Mutant Biophysics Arthritis 02 engineering and technology Osteoarthritis 03 medical and health sciences Mice Endopeptidases medicine Animals General Materials Science Aggrecans Aggrecan Aggrecanase medicine.diagnostic_test Cartilage Wild type Surfaces and Interfaces General Chemistry 021001 nanoscience & nanotechnology medicine.disease Elasticity 030104 developmental biology medicine.anatomical_structure Mutation 0210 nano-technology Biotechnology Interleukin-1 |
| Zdroj: | The European physical journal. E, Soft matter. 40(8) |
| ISSN: | 1292-895X |
| Popis: | A key event in arthritis pathogenesis is the degradation of aggrecan, the major component in articular cartilage. In this work, we investigate the effects of stimulated aggrecanolysis on the morphological and nanomechanical properties of cartilage harvested from wild-type mice and aggrecanase-resistant mutant mice named "Jaffa". The cartilages were native or were subjected to stimulated aggrecanolysis by interleukin-1[Formula: see text] (IL-1[Formula: see text]) treatment. The nanoscale morphological and mechanical properties of the sectioned cartilages were measured by using a sharp probe by atomic force microscopy (AFM). The IL-1[Formula: see text] treatment resulted in a higher nanoroughess and stiffness of the cartilage from wild-type mice. However, the same treatment did not lead to any measurable change in the nanoroughness or stiffness of the cartilage from mutant mice Jaffa. This suggests that blocking aggrecanolysis by genetic modification has created the stability in the structures and mechanical properties of the cartilage at nanoscale. The present study provides insight into the mechanism of aggrecan degradation, which can complement the examination by biochemical and histological techniques. |
| Databáze: | OpenAIRE |
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