Microtubule Dynamics during Infection-Related Morphogenesis of Colletotrichum lagenarium
| Autor: | Yoshitaka Takano, Tetsuro Okuno, Eriko Oshiro |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Recombinant Fusion Proteins
Green Fluorescent Proteins Molecular Sequence Data Gene Expression Spindle Apparatus Biology Microtubules Microbiology Spindle pole body chemistry.chemical_compound Tubulin Microtubule Cell cortex Morphogenesis Genetics Amino Acid Sequence Mitosis Phylogeny Cell Nucleus Appressorium Sequence Homology Amino Acid fungi Benomyl Cell biology Luminescent Proteins chemistry Interphase Mitosporic Fungi Astral microtubules Cell Division |
| Zdroj: | Fungal Genetics and Biology. 34:107-121 |
| ISSN: | 1087-1845 |
| DOI: | 10.1006/fgbi.2001.1293 |
| Popis: | Using a green fluorescent protein (GFP)–tubulin fusion protein, we have investigated the dynamic rearrangement of microtubules during appressorium formation of Colletotrichum lagenarium. Two α-tubulin genes of C. lagenarium were isolated, and GFP–α-tubulin protein was expressed in this fungus. The strain expressing the fusion protein formed fluorescent filaments that were disrupted by a microtubule-depolymerizing drug, benomyl, demonstrating successful visualization of microtubules. In preincubated conidia, GFP-labeled interphase microtubules, showing random orientation, were observed. At conidial germination, microtubules oriented toward a germination site. At nuclear division, when germ tubes had formed appressoria, mitotic spindles appeared inside conidia followed by disassembly of interphase microtubules. Remarkably, time-lapse views showed that interphase microtubules contact a microtubule-associated center at the cell cortex of conidia that is different from a nuclear spindle pole body (SPB) before their disassembly. Duplicated nuclear SPBs separately moved toward conidium and appressorium accompanied by astral microtubule formation. Benomyl treatment caused movement of both daughter nuclei into 70% of appressoria and affected appressorium morphogenesis. In conidia elongating hyphae without appressoria, microtubules showed polar elongation which is distinct from their random orientation inside appressoria. |
| Databáze: | OpenAIRE |
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