The in vitro effect of leucocyte α-interferon on human myeloma cells in a semisolid agar culture system
| Autor: | Gunilla Brenning |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Melphalan
food.ingredient Bone Marrow Cells Stimulation Pharmacology Biology Colony-Forming Units Assay food Interferon medicine Humans Agar Clonogenic assay Cells Cultured Multiple myeloma Hematology medicine.disease In vitro Culture Media medicine.anatomical_structure Interferon Type I Immunology Neoplastic Stem Cells Bone marrow Multiple Myeloma medicine.drug |
| Zdroj: | Scandinavian Journal of Haematology. 35:178-185 |
| ISSN: | 0036-553X |
| DOI: | 10.1111/j.1600-0609.1985.tb01568.x |
| Popis: | 73 bone marrow samples from a total of 50 patients with multiple myeloma (MM) were tested for interferon (IFN) sensitivity in the human tumour colony assay (HTCA). 16 evaluable samples were obtained from untreated patients, 10 from patients during melphalan and prednisone treatment and 6 from patients on IFN treatment or after withdrawal of IFN. The sensitivity to IFN was individually distributed between the bone marrow samples from patients of alle 3 groups and a tendency towards IFN resistance could be found in serial assays from patients during MP-treatment and after IFN withdrawal. Stimulation of growth was found in 31% of the cultures, usually at lower doses of IFN (10–100 U/ml) while higher doses (400–4000 U/ml) inhibited growth in 75% of the assays. |
| Databáze: | OpenAIRE |
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