Hybridization of binary monolayers of single stranded oligonucleotides and short blocking molecules
| Autor: | Inger Vikholm-Lundin, Heidi Fiegl, Sophia Apostolidou, Sanna Auer, Tony Munter |
|---|---|
| Rok vydání: | 2009 |
| Předmět: |
Base pair
Analytical chemistry law.invention chemistry.chemical_compound SDG 3 - Good Health and Well-being law Monolayer Materials Chemistry Surface plasmon resonance hybridization Polymerase chain reaction oligonucleotides Oligonucleotide self-assembly Surfaces and Interfaces Amplicon Condensed Matter Physics Surfaces Coatings and Films Crystallography chemistry immobilization Biosensor surface plasmon resonance DNA |
| Zdroj: | Vikholm-Lundin, I, Auer, S, Munter, T, Fiegl, H & Apostolidou, S 2009, ' Hybridization of binary monolayers of single-stranded oligonucleotides and short blocking molecules ', Surface Science, vol. 603, no. 4, pp. 620-624 . https://doi.org/10.1016/j.susc.2008.12.026 |
| ISSN: | 0039-6028 |
| Popis: | We have studied the immobilization of single stranded (ss) DNA oligonucleotides of 16-27 base pairs on gold. The oligonucleotides were thiol-modified (SH-ssDNA) or disulfide-modified via a dimethoxytrityl-group (DMT-S-S-ssDNA). Immobilization was performed by adsorption of the probes on the gold surface for 10-15 min, a time within which saturation coverage was obtained for both thiol- and disulfide-modified probes. Hereafter the layer was post-treated with hydroxyalkyl substituted lipoamides also for a time of 10-15 min. The surface density of layers with shorter probes (16-18 met) was twice (2.4 +/- 0.2 x 10(13) probes/cm(2)) that of the longer probes (25-27 met) as studied with surface plasmon resonance. Hybridization of single stranded polymerase chain reaction (PCR) amplified products with a length above 300 base pairs gave a very low hybridization response. For amplicons with about 100 base pairs the response was high. The surface coverage was comparable to that of complementary ssDNA binding (3.0 x 10(12) strands/cm(2)). Surfaces made from SH-ssDNA showed a 30% higher hybridization response than surfaces made from DMT-S-S-ssDNA. The PCR amplified products used are of relevance in breast cancer diagnosis. The results clearly demonstrate that the single stranded PCR products might be used in label-free cancer diagnostics. (C) 2009 Elsevier B.V. All rights reserved. |
| Databáze: | OpenAIRE |
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