EasyNAT MTC assay: A simple, rapid, and low-cost cross-priming amplification method for the detection of mycobacterium tuberculosis suitable for point-of-care testing
| Autor: | Guanglu Jiang, Liping Zhao, Hairong Huang, Zhuman Zhang, Jian Du, Feng Wang, Junnan Jia, Lingling Dong, Xia Yu, Tao Liu, Yi Xue |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
medicine.medical_specialty
Tuberculosis Epidemiology diagnosis Point-of-care testing Immunology Xpert MTB/RIF Cross-priming Diagnostic tools Microbiology Sensitivity and Specificity Mycobacterium tuberculosis Bacterial Proteins Pulmonary tuberculosis Virology Drug Discovery Medicine Humans Intensive care medicine Tuberculosis Pulmonary point of care testing biology business.industry Sputum General Medicine medicine.disease biology.organism_classification Infectious Diseases Ustar EasyNAT MTC assay Molecular Diagnostic Techniques Point-of-Care Testing Parasitology business Nucleic Acid Amplification Techniques Research Article |
| Zdroj: | Emerging Microbes & Infections article-version (VoR) Version of Record |
| ISSN: | 2222-1751 |
| Popis: | More sensitive, rapid, and affordable diagnostic tools for pulmonary tuberculosis (PTB) are urgently needed. This study aimed to assess the performance of EasyNAT MTC (abbreviation: EasyNAT) (Ustar Biotechnologies, China), a novel isothermal amplification method with a turnaround time of less than two hours that requires a few manual steps to process the sputum. Sputum samples from 249 patients with suspected PTB were subjected to smear, culture, Xpert MTB/RIF (Cepheid, USA) and EasyNAT assay testing. Of the 169 PTB patients, EasyNAT detected more PTB patients than Xpert (72.19% vs. 61.54%, P |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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