Identification of GLA/SE as an effective adjuvant for the induction of robust humoral and cell-mediated immune responses to EBV-gp350 in mice and rabbits
| Autor: | Jennifer Woo, Rui Lin, Michael P. McCarthy, Jason D. Marshall, Eileen Rao, Darren S. Heeke |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
CD4-Positive T-Lymphocytes Herpesvirus 4 Human medicine.medical_treatment T cell Monophosphoryl Lipid A Antibodies Viral Virus Viral Matrix Proteins 03 medical and health sciences Mice Immune system Adjuvants Immunologic Glucosides medicine Animals Neutralizing antibody Immunity Cellular General Veterinary General Immunology and Microbiology biology Public Health Environmental and Occupational Health Antibody titer Viral Vaccines Virology Antibodies Neutralizing Immunity Humoral Mice Inbred C57BL Toll-Like Receptor 4 030104 developmental biology Infectious Diseases medicine.anatomical_structure Lipid A Immunoglobulin G Immunology biology.protein Molecular Medicine Female Rabbits Antibody Adjuvant |
| Zdroj: | Vaccine. 34(23) |
| ISSN: | 1873-2518 |
| Popis: | Childhood infection with Epstein-Barr virus (EBV) is often asymptomatic and may result in mild flu-like symptoms, but exposure during adolescence and young adulthood can lead to acute infectious mononucleosis (AIM) with a pathology characterized by swollen lymph nodes, sore throat, and severe fatigue lasting weeks or months. A vaccine targeting the envelope glycoprotein gp350 adjuvanted with aluminum hydroxide complexed with the TLR4 agonist monophosphoryl lipid A (MPLA) achieved a 78% reduction in AIM incidence in a small phase II trial of college-age individuals, but development of this vaccine was halted by the manufacturer. Here, we report the evaluation in mice and rabbits of an EBV-gp350 vaccine combined with an adjuvant composed of the synthetic TLR4 agonist glucopyranosyl lipid A (GLA) integrated into stable emulsion (SE). In mice, GLA/SE-adjuvanted gp350 generated high IgG titers (both IgG1 and IgG2a/c subtypes), elevated EBV-neutralizing antibody titers, and robust poly-functional anti-gp350 CD4(+) T cell responses. In addition, GLA/SE routinely demonstrated superior performance over aluminum hydroxide in all immunological readouts, including induction of durable neutralizing antibody titers out to at least 1 year post-vaccination. Both components of the GLA/SE adjuvant were found to be required to get optimal responses in both arms of the immune response: specifically, SE for neutralizing antibodies and GLA for induction of T cell responses. Furthermore, this vaccine also elicited high neutralizing antibody titers in a second species, rabbit. These promising results suggest that clinical development of a vaccine comprised of EBV-gp350 plus GLA/SE has the potential to prevent AIM in post-adolescents. |
| Databáze: | OpenAIRE |
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