Antioxidant treatment reverts increased arterial basal tone and oxidative stress in nephrectomized (5/6) hypertensive rats
| Autor: | Rodrigo O Maranon, María Sofía Karbiner, Claudio M Joo Turoni, María Peral de Bruno, Nicolás Martín Salas |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: |
BASAL TONE
lcsh:Diseases of the circulatory (Cardiovascular) system medicine.medical_specialty Mean arterial pressure CIENCIAS MÉDICAS Y DE LA SALUD Article Subject Renal function medicine.disease_cause Nitric oxide chemistry.chemical_compound Basal (phylogenetics) Internal medicine medicine.artery Internal Medicine Medicine Endothelial dysfunction Aorta NEPHRECTOMY business.industry purl.org/becyt/ford/3.1 [https] Bioquímica y Biología Molecular medicine.disease Medicina Básica Endocrinology chemistry lcsh:RC666-701 purl.org/becyt/ford/3 [https] business Oxidative stress NITRIC OXIDE Research Article Kidney disease |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET International Journal of Hypertension International Journal of Hypertension, Vol 2013 (2013) |
| Popis: | Nonischemic 5/6 nephrectomized rat (NefR) is a model of chronic kidney disease. However, little is known about vascular dysfunction and its relation with hypertension in NefR. Aims. To evaluate possible alterations of endothelial function, NObioavailability, and basal tone in aorta from NefR and the role of oxidative stress. Sprague Dawley rats were divided into sham rats (SR), NefR, and NefR treated with tempol (NefR-T). Mean arterial pressure (MAP) and renal function were determined. In isolated aortic rings the following was measured: 1-endothelial function, 2-basal tone, 3-NO levels, 4-membrane potential (MP), and 5-oxidative stress. NefR increased MAP (SR: 119 ± 4 mmHg; =7; NefR: 169 ± 6; =8; < 0.001). Tempol did not modify MAP (NefR-T: 168 ± 10; =6; < 0.001). NefR showed endothelial dysfunction, increased basal tone and decreased NO levels (SR: 32 ± 2 nA; =7, NefR: 10 ± 2; =8; < 0.001). In both in vitro and in vivo tempol improves basal tone, NO levels, and MP. Oxidative stress in NefR was reverted in NefR-T. We described, for the first time, that aorta from NefR presented increased basal tone related to endothelial dysfunction and decreased NO-bioavailability. The fact that tempol improves NO-contents and basal tone, without decrease MAP, indicates that oxidative stress could be implicated early and independently to hypertension, in the vascular alterations. Fil: Marañón, Rodrigo Oscar. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina. University Of Mississippi; Estados Unidos Fil: Joo Turoni, Claudio Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina Fil: Karbiner, María Sofía. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina Fil: Salas, Nicolás Martín. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina Fil: Peral, Maria de Los Angeles. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucuman. Instituto Superior de Investigaciones Biológicas. Universidad Nacional de Tucuman. Instituto Superior de Investigaciones Biologicas; Argentina |
| Databáze: | OpenAIRE |
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