Artepillin C Derived from Propolis Induces Neurite Outgrowth in PC12m3 Cells via ERK and p38 MAPK Pathways
| Autor: | Sueo Eguchi, Hidefumi Maeda, Kenji Kawamura, Fumika Aramaki, Shima Doi, Fukumi Hiragami, Noboru Horie, Yoshihisa Koike, Junichi Akiyama, Ken Hashimoto, Yoshio Kano, Hirotoshi Motoda |
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| Rok vydání: | 2008 |
| Předmět: |
MAPK/ERK pathway
Neurite MAP Kinase Signaling System Pyridones p38 mitogen-activated protein kinases Apoptosis PC12 Cells p38 Mitogen-Activated Protein Kinases Biochemistry Propolis Mice Cellular and Molecular Neuroscience Enzyme activator Alkaloids Anti-Infective Agents Neurites Animals Humans Enzyme Inhibitors Nucleic Acid Synthesis Inhibitors Mitogen-Activated Protein Kinase 1 Mitogen-Activated Protein Kinase 3 Molecular Structure Phenylpropionates biology Chemistry General Medicine Rats Cell biology Enzyme Activation Nerve growth factor Mitogen-activated protein kinase biology.protein Phosphorylation |
| Zdroj: | Neurochemical Research. 33:1795-1803 |
| ISSN: | 1573-6903 0364-3190 |
| DOI: | 10.1007/s11064-008-9633-9 |
| Popis: | We investigated whether artepillin C, a major component of Brazilian propolis, acts as a neurotrophic-like factor in rat PC12m3 cells, in which nerve growth factor (NGF)-induced neurite outgrowth is impaired. When cultures of PC12m3 cells were treated with artepillin C at a concentration of 20 microM, the frequency of neurite outgrowth induced by artepillin C was approximately 7-fold greater than that induced by NGF alone. Artepillin C induced-neurite outgrowth of PC12m3 cells was inhibited by the ERK inhibitor U0126 and by the p38 MAPK inhibitor SB203580. Although artepillin C-induced p38 MAPK activity was detected in PC12m3 cells, phosphorylation of ERK induced by artepillin C was not observed. On the other hand, artepillin C caused rapid activation of ERK and the time course of the activation was similar to that induced by NGF treatment in PC12 parental cells. However, NGF-induced neurite outgrowth was inhibited by artepillin C treatment. Interestingly, inhibition of ERK by U0126 completely prevented artepillin C-induced p38 MAPK phosphorylation of PC12m3 cells. These findings suggest that artepillin C-induced activation of p38 MAPK through the ERK signaling pathway is responsible for the neurite outgrowth of PC12m3 cells. |
| Databáze: | OpenAIRE |
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