Pharmacological analysis of the neutrophil migration induced by D. rostrata lectin: involvement of cytokines and nitric oxide
| Autor: | Ana Maria Sampaio Assreuy, Fernando Q. Cunha, Benildo Sousa Cavada, Marcus Raimundo Vale, Daniela Dal-Secco, Jozi G. Figueiredo, Flávio S. Bitencourt, Priscila P. Silvestre, Kyria S. Nascimento, Mário Rogério Lima Mota, Raquel G. Benevides, T.R. Moura, Nylane M.N. Alencar, Cibele N. Aguiar |
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| Rok vydání: | 2009 |
| Předmět: |
Male
Leukocyte migration medicine.medical_specialty Neutrophils medicine.medical_treatment Inflammation Biology Toxicology Nitric Oxide Peritoneal cavity Cell Movement Internal medicine Lectins medicine Animals Rats Wistar Plant Extracts Peritoneal fluid Chemotaxis Neutrophil extracellular traps Mast cell Molecular biology Rats medicine.anatomical_structure Endocrinology Cytokine Dioclea Cytokines medicine.symptom |
| Zdroj: | Toxicon : official journal of the International Society on Toxinology. 54(6) |
| ISSN: | 1879-3150 |
| Popis: | In the present study, we investigated the involvement of resident cell and inflammatory mediators in the neutrophil migration induced by chemotactic activity of a glucose/mannose-specific lectin isolated from Dioclea rostrata seeds (DrosL). Rats were injected i.p. with DrosL (125-1000 microg/cavity), and at 2-96 h thereafter the leukocyte counts in peritoneal fluid were determined. DrosL-induced a dose-dependent neutrophil migration accumulation, which reached maximal response at 24 h after injection and declines thereafter. The carbohydrate ligand nearly abolished the neutrophil influx. Pre-treatment of peritoneal cavities with thioglycolate which increases peritoneal macrophage numbers, enhanced neutrophil migration induced by DrosL by 303%. However, the reduction of peritoneal mast cell numbers by treatment of the cavities with compound 48/80 did not modify DrosL-induced neutrophil migration. The injection into peritoneal cavities of supernatants from macrophage cultures stimulated with DrosL (125, 250 and 500 microg/ml) induced neutrophil migration. In addition, DrosL treatment induced cytokines (TNF-alpha, IL-1beta and CINC-1) and NO release into the peritoneal cavity of rats. Finally, neutrophil chemotaxis assay in vitro showed that the lectin (15 and 31 microg/ml) induced neutrophil chemotaxis by even 180%. In conclusion, neutrophil migration induced by D. rostrata lectin occurs by way of the release of NO and cytokines such as IL-1beta, TNF-alpha and CINC-1. |
| Databáze: | OpenAIRE |
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