Potassium channels-mediated electrophysiologic responses are inhibited by cytosolic phospholipase A2α ablation
| Autor: | Na Wang, Ying-Hong Hu, Li-Da Su |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Patch-Clamp Techniques Potassium Channels Action Potentials Mice Transgenic In Vitro Techniques Hippocampal formation Phospholipase Apamin Hippocampus Mice 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Potassium Channel Blockers medicine Animals Patch clamp 4-Aminopyridine Pyramidal Cells General Neuroscience Neurotoxicity Potassium channel blocker medicine.disease Electric Stimulation Potassium channel Electrophysiological Phenomena Cell biology 030104 developmental biology nervous system chemistry Calcium Paxillin 030217 neurology & neurosurgery 2S Albumins Plant medicine.drug |
| Zdroj: | NeuroReport. 29:59-64 |
| ISSN: | 0959-4965 |
| Popis: | Cytosolic phospholipase A2α (cPLA2α) is implicated in the progression of excitotoxic neuronal injury and cerebral ischemia. Previous work suggests that cPLA2α increases aberrant electrophysiologic events through attenuating K channel functions. Nevertheless, which K channels are affected by cPLA2α needs to be determined. Here we examined K channels-mediated electrophysiologic responses in hippocampal CA1 pyramidal neurons from wild-type and cPLA2α mice using simultaneous patch-clamp recording and confocal Ca imaging. After the exposure to the blockers of Ca-sensitive and A-type K channels, all CA1 neurons developed spike broadening and increased dendritic Ca transients. These effects were occluded in CA1 neurons from cPLA2α mice. Therefore, cPLA2α modulates the functions of Ca-sensitive and A-type K channels in neurotoxicity. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|