Reversion of the effects of a threonine deaminase regulatory mutant by a mutation in ilvH in Escherichiacoli K-12
| Autor: | Phillip A. Singer, Luther S. Williams, Mark Levinthal |
|---|---|
| Rok vydání: | 1984 |
| Předmět: |
Mutation
Operon Mutant Structural gene Biophysics Reversion Cell Biology Biology medicine.disease_cause Biochemistry Molecular biology Amino Acyl-tRNA Synthetases Kinetics Species Specificity Threonine Dehydratase Genes Bacterial Genes Regulator Transfer RNA Escherichia coli medicine Threonine Molecular Biology Gene |
| Zdroj: | Biochemical and Biophysical Research Communications. 118:270-277 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/0006-291x(84)91096-9 |
| Popis: | In a strain carrying an ilvA538 mutation, the ilvGEDA operon expression is decreased (hyperattenuated) and the activity and/or expression of isoleucyl- and valyl- tRNA synthetases is decreased. We have isolated two revertants of ilvA538 owing to mutations in the ilvH gene, whose product is acetohydroxy acid synthase III. The regulatory properties of these revertants are consistent with a dual role for threonine deaminase as an effector of the ilvGEDA operon and the isoleucyl- and valyl- tRNA synthetase structural genes. |
| Databáze: | OpenAIRE |
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