Analysis of Y-P30/Dermcidin expression and properties of the Y-P30 peptide

Autor: Mikhaylova, Marina, Schumacher, A., Borutzki, C., Neumann, J.R., Macharadze, T., El-Mousleh, T., Wahle, P., Zenclussen, A.C., Kreutz, M.R., Sub Cell Biology, Celbiologie
Přispěvatelé: Sub Cell Biology, Celbiologie
Jazyk: angličtina
Rok vydání: 2014
Předmět:
animal diseases
viruses
Placenta
Gene Expression
Peptide
Pregnancy
Chlorocebus aethiops
Cells
Cultured
Progesterone
Medicine(all)
chemistry.chemical_classification
Microscopy
Confocal
Estradiol
Reverse Transcriptase Polymerase Chain Reaction
General Medicine
Transfection
medicine.anatomical_structure
COS Cells
Female
alpha-Fetoproteins
Research Article
Adult
Syndecans
Blotting
Western
Green Fluorescent Proteins
Biology
Peripheral blood mononuclear cell
General Biochemistry
Genetics and Molecular Biology
Young Adult
Immune system
parasitic diseases
medicine
Dermcidin Y-P30
Animals
Humans
Secretion
Biochemistry
Genetics and Molecular Biology(all)
urogenital system
HEK 293 cells
Molecular biology
In vitro
Pregnancy Trimester
First
HEK293 Cells
chemistry
PBMCs
Leukocytes
Mononuclear
Syndecan-3
Syndecan-4
Peptides
Zdroj: BMC Research Notes
BMC Research Notes, 7. BioMed Central
ISSN: 1756-0500
DOI: 10.1186/1756-0500-7-400
Popis: Background: The survival promoting peptide Y-P30 has a variety of neuritogenic and neuroprotective effects in vitro and in vivo. In previous work we reported the expression of Y-P30/dermcidin in maternal peripheral blood mononuclear cells (PBMCs) and the transport of the protein to the fetal brain. In this study we analyzed hormonal regulation of Y-P30 in human immune cells and expression of Y-P30 in the placenta. We further studied the stability and secretion of the Y-P30 peptide. Results: We found indications that Y-P30 might be produced in human placenta. The Y-P30 mRNA was rarely found in isolated human PBMCs and alpha-feto-protein, human chorionic gonadotropin as well as estradiol combined with progesterone could not induce Y-P30 expression. Y-P30 was found to be extraordinarily stable; therefore, contamination with the peptide and the Y-P30/Dermcidin precursor mRNA is a serious concern in experiments looking at the expression of Y-P30/Dermcidin. In cultured cell lines and primary neurons we found that Y-P30 could be released, but neuronal uptake of Y-P30 was not observed. Conclusions: Our data suggest that a source of Y-P30 apart from eccrine glands might be the placenta. The peptide can be secreted together with the signaling peptide and it might reach the fetal brain where it can exert its neuritogenic functions by binding to neuronal membranes.
Databáze: OpenAIRE
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