Human cardiosphere-seeded gelatin and collagen scaffolds as cardiogenic engineered bioconstructs

Autor: Andrea Barbetta, Alessandro Giacomello, Mariella Dentini, Francesco Angelini, Elisa Messina, G. Rizzitelli, Vittoria Ionta, Isotta Chimenti, Elvira Forte, Giacomo Frati, Olivier Schussler, Roberto Gaetani
Přispěvatelé: Department of Molecular Medicine, Institut Pasteur, Fondation Cenci Bolognetti - Istituto Pasteur Italia, Fondazione Cenci Bolognetti, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], Department of Chemistry University 'La Sapienza', Università degli Studi di Roma 'La Sapienza' = Sapienza University [Rome], Department of Biotechnology and Medical - Surgical Sciences, Department of Angiocardioneurology, ' Neuromed ' (IRCCS), Department of Cardiovascular Surgery, Geneva University Hospital (HUG), Department of Cardiovascular Fisiopathology, Anesthesiology and General Surgery, This project was funded by Italian MIUR and Pasteur Institute,Cenci-Bolognetti Foundation. IC and RG were supported by PasteurInstitute, Cenci-Bolognetti Foundation fellowships.
Jazyk: angličtina
Rok vydání: 2011
Předmět:
collagen
[SDV.BIO]Life Sciences [q-bio]/Biotechnology
Cell
Fluorescent Antibody Technique
MESH: Spheroids
Cellular
MESH: X-Ray Microtomography
030204 cardiovascular system & hematology
Matrix (biology)
Gelatin
MESH: Tissue Engineering
cardiospheres
Extracellular matrix
0302 clinical medicine
Tissue engineering
MESH: Collagen
MESH: Tissue Scaffolds
MESH: Animals
[INFO.INFO-BT]Computer Science [cs]/Biotechnology
MESH: Fluorescent Antibody Technique
Cells
Cultured
0303 health sciences
Tissue Scaffolds
MESH: Molecular Weight
Cell migration
MESH: Gene Expression Regulation
MESH: Cattle
Phenotype
medicine.anatomical_structure
MESH: Cell Survival
Mechanics of Materials
MESH: Gelatin
Rheology
MESH: Cells
Cultured
Materials science
food.ingredient
MESH: Myocardium
MESH: Microscopy
Electron
Scanning
Cell Survival
Biophysics
Bioengineering
MESH: Phenotype
Biomaterials
gelatin
03 medical and health sciences
cardiac tissue engineering
food
MESH: Rheology
Spheroids
Cellular
MESH: Cell Proliferation
medicine
Animals
Humans
Cell Proliferation
030304 developmental biology
MESH: Humans
Tissue Engineering
Cell growth
Myocardium
Histology
X-Ray Microtomography
Molecular Weight
Gene Expression Regulation
Microscopy
Electron
Scanning
Ceramics and Composites
Cattle
Biomedical engineering
Zdroj: Biomaterials
Biomaterials, Elsevier, 2011, 32 (35), pp.9271-81. ⟨10.1016/j.biomaterials.2011.08.049⟩
ISSN: 0142-9612
DOI: 10.1016/j.biomaterials.2011.08.049⟩
Popis: International audience; Cardiac tissue engineering (CTE) aims at regenerating damaged myocardium by combining cells to a biocompatible and/or bioactive matrix. Collagen and gelatin are among the most suitable materials used today for CTE approaches. In this study we compared the structural and biological features of collagen (C-RGD) or gelatin (G-FOAM)-based bioconstructs, seeded with human adult cardiac progenitor cells in the form of cardiospheres (CSps). The different morphology between C-RGD (fibrous ball-of-thread-like) and G-FOAM (trabecular sponge-like) was evidenced by SEM analysis and X-ray micro-tomography, and was reflected by their different mechanical characteristics. Seeded cells were viable and proliferating after 1 week in culture, and a reduced expression of cell-stress markers versus standard CSp culture was detected by realtime PCR. Cell engraftment inside the scaffolds was assessed by SEM microscopy and histology, evidencing more relevant cell migration and production of extracellular matrix in C-RGD versus G-FOAM. Immunofluorescence and realtime PCR analysis showed down-regulation of vascular and stemness markers, while early-to-late cardiac markers were consistently and significantly upregulated in G-FOAM and C-RGD compared to standard CSps culture, suggesting selective commitment towards cardiomyocytes. Overall our results suggest that CSp-bioconstructs have suitable mechanical properties and improved survival and cardiogenic properties, representing promising tools for CTE.
Databáze: OpenAIRE
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