Micro-RNA-Regulated SQUAMOSA-PROMOTER BINDING PROTEIN-LIKE (SPL) Gene Expression and Cytokinin Accumulation Distinguish Early-Developing Male and Female Inflorescences in Oil Palm (Elaeis guineensis)
| Autor: | Tregear, James, Richaud, Frédérique, Collin, Myriam, Esbelin, Jennifer, Parrinello, Hugues, Cochard, Benoît, Nodichao, Leifi, Morcillo, Fabienne, Adam, Hélène, Jouannic, Stefan |
|---|---|
| Přispěvatelé: | Diversité, adaptation, développement des plantes (UMR DIADE), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Université de Montpellier (UM), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Institut de Génomique Fonctionnelle (IGF), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM), PalmElit SAS, Institut National de Recherche Agricole du Bénin (INRAB), INRAB |
| Rok vydání: | 2022 |
| Předmět: |
inflorescence
branching sexual differentiation oil palm miRNA SQUAMOSA PROMOTER-BINDING PROTEIN Ecology food and beverages F62 - Physiologie végétale - Croissance et développement Plant Science Différenciation sexuelle F30 - Génétique et amélioration des plantes ARN Ramification Gène [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Elaeis guineensis Ecology Evolution Behavior and Systematics |
| Zdroj: | Plants Plants, 2022, 11 (5), ⟨10.3390/plants11050685⟩ Plants; Volume 11; Issue 5; Pages: 685 |
| ISSN: | 2223-7747 |
| DOI: | 10.3390/plants11050685 |
| Popis: | International audience; Sexual differentiation of inflorescences and flowers is important for reproduction and affects crop plant productivity. We report here on a molecular study of the process of sexual differentiation in the immature inflorescence of oil palm (Elaeis guineensis). This species is monoecious and exhibits gender diphasy, producing male and female inflorescences separately on the same plant in alternation. Three main approaches were used: small RNA-seq to characterise and study the expression of miRNA genes; RNA-seq to monitor mRNA accumulation patterns; hormone quantification to assess the role of cytokinins and auxins in inflorescence differentiation. Our study allowed the characterisation of 30 previously unreported palm MIRNA genes. In differential gene and miRNA expression studies, we identified a number of key developmental genes and miRNA-mRNA target modules previously described in relation to their developmental regulatory role in the cereal panicle, notably the miR156/529/535-SQUAMOSA PROMOTER-BINDING PROTEIN-LIKE (SPL) gene regulatory module. Gene enrichment analysis highlighted the importance of hormone-related genes, and this observation was corroborated by the detection of much higher levels of cytokinins in the female inflorescence. Our data illustrate the importance of branching regulation within the developmental window studied, during which the female inflorescence, unlike its male counterpart, produces flower clusters on new successive axes by sympodial growth. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|