Molecular characterization of a hepatitis E virus isolate from Namibia
| Autor: | Leonard N. Binn, Junkun He, Bruce L. Innis, Curtis G. Hayes, Margaretha Isaäcson, Sergei A. Tsarev, John Frean |
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| Rok vydání: | 2000 |
| Předmět: |
Genotype
Hepatitis B virus DNA polymerase viruses Endocrinology Diabetes and Metabolism Clinical Biochemistry Biology medicine.disease_cause law.invention Open Reading Frames Capsid Hepatitis E virus law Consensus Sequence parasitic diseases medicine Humans Pharmacology (medical) Molecular Biology Phylogeny Polymerase chain reaction Molecular epidemiology Reverse Transcriptase Polymerase Chain Reaction Biochemistry (medical) virus diseases Outbreak Sequence Analysis DNA Cell Biology General Medicine medicine.disease Hepatitis E Namibia Virology digestive system diseases Viral hepatitis Oncovirus |
| Zdroj: | Journal of Biomedical Science. 7:334-338 |
| ISSN: | 1423-0127 1021-7770 |
| DOI: | 10.1007/bf02253253 |
| Popis: | Hepatitis E virus (HEV) causes sporadic and epidemic acute viral hepatitis in many developing countries. In Africa, hepatitis E has been documented by virus detection (reverse transcriptase polymerase chain reaction, RT-PCR) in Egypt, Chad, Algeria, Morocco and Tunisia. Cases of presumptive hepatitis E also have been documented by detection of antibody to HEV in the Sudan, Kenya, Ethiopia, Somalia, Djibouti and South Africa. Recently, we reported the recovery of 9 isolates of HEV from feces collected during an outbreak of jaundice in Namibia. These specimens were stored frozen for many years at the South African Institute for Medical Research awaiting new methods to determine the etiology of jaundice. HEV genomic sequences were detected by antigen-capture RT-PCR with primers that amplified 2 independent regions of the HEV genome (ORF-2 and ORF-3). To further characterize the HEV 83-Namibia isolates, we determined the nucleotide (nt) sequence of the 3' end of the capsid gene (296 of 1, 980 nt in ORF-2) and ORF-3 for 1 isolate. The capsid gene sequence shared 86% identity with the prototype Burma strain and up to 96% identity with other African strains at the (nt) level, and 99% identity with Burma or other Africa strains at the amino acid level. A 188 (nt) fragment amplified from ORF-3 was also highly homologous to other HEV but was too short for meaningful comparison. Phylogenetic analysis indicated that HEV 83-Namibia is closely related to other African isolates, and differs from Burmese, Mexican and Chinese HEV. These data link the HEV causing the 1983 Namibia outbreak to more recent HEV transmission in northern and sub-Saharan Africa, suggesting this subgenotype of HEV is firmly established throughout the continent. |
| Databáze: | OpenAIRE |
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