Global analysis of Chlorella variabilis NC64A mRNA profiles during the early phase of Paramecium bursaria chlorella virus-1 infection

Autor: James R. Gurnon, James L. Van Etten, Adrien Jeanniard, Guillaume Blanc, Yuannan Xia, Janet M. Rowe, David D. Dunigan
Jazyk: angličtina
Rok vydání: 2014
Předmět:
RNA viruses
0106 biological sciences
Time Factors
Gene Expression
lcsh:Medicine
Plant Science
Chlorella
Virus Replication
Biochemistry
01 natural sciences
chemistry.chemical_compound
Plant Microbiology
Viral classification
Gene Expression Regulation
Plant
Transcription (biology)
Nucleic Acids
RNA polymerase
Molecular Cell Biology
Phycodnaviridae
lcsh:Science
0303 health sciences
education.field_of_study
Multidisciplinary
Genomics
DNA-Directed RNA Polymerases
Plants
Host-Pathogen Interaction
RNA
Plant
Host-Pathogen Interactions
Phycology
Research Article
Ribosomal Proteins
Algae
Population
Plant Pathogens
Marine Biology
Cyclopentanes
Biology
Microbiology
Virus
03 medical and health sciences
Virology
Genetics
Oxylipins
RNA
Messenger
education
Gene
030304 developmental biology
Messenger RNA
Gene Expression Profiling
Algal Proteins
lcsh:R
RNA
Plant Pathology
Molecular biology
Viral Replication
Protein Subunits
chemistry
Viral replication
DNA
Viral
Spliceosomes
lcsh:Q
Genome Expression Analysis
Transcriptome
010606 plant biology & botany
Zdroj: PLoS ONE, Vol 9, Iss 3, p e90988 (2014)
PLoS ONE
ISSN: 1932-6203
Popis: The PBCV-1/Chlorella variabilis NC64A system is a model for studies on interactions between viruses and algae. Here we present the first global analyses of algal host transcripts during the early stages of infection, prior to virus replication. During the course of the experiment stretching over 1 hour, about a third of the host genes displayed significant changes in normalized mRNA abundance that either increased or decreased compared to uninfected levels. The population of genes with significant transcriptional changes gradually increased until stabilizing at 40 minutes post infection. Functional categories including cytoplasmic ribosomal proteins, jasmonic acid biosynthesis and anaphase promoting complex/cyclosomes had a significant excess in upregulated genes, whereas spliceosomal snRNP complexes and the shikimate pathway had significantly more down-regulated genes, suggesting that these pathways were activated or shut-down in response to the virus infection. Lastly, we examined the expression of C. varibilis RNA polymerase subunits, as PBCV-1 transcription depends on host RNA polymerases. Two subunits were up-regulated, RPB10 and RPC34, suggesting that they may function to support virus transcription. These results highlight genes and pathways, as well as overall trends, for further refinement of our understanding of the changes that take place during the early stages of viral infection.
Databáze: OpenAIRE
Externí odkaz: