New silver complexes with bioactive glycine and nicotinamide molecules – Characterization, DNA binding, antimicrobial and anticancer evaluation
| Autor: | Z. Vargová, Michaela Rendošová, Helena Paulíková, Mária Vilková, Danica Sabolová, Júlia Kudláčová, Miroslav Almáši, Michal Dušek, Daniela Hudecová, Juraj Kuchár, Dáša Bobáľová, Štefan Levoča, Veronika Helebrandtová |
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| Rok vydání: | 2017 |
| Předmět: |
Niacinamide
Circular dichroism Silver Cell Survival Stereochemistry Glycine Antineoplastic Agents Crystallography X-Ray 010402 general chemistry 01 natural sciences Biochemistry Inorganic Chemistry Inhibitory Concentration 50 Mice chemistry.chemical_compound Anti-Infective Agents Coordination Complexes Cell Line Tumor Animals chemistry.chemical_classification Quenching (fluorescence) Bacteria biology Nicotinamide 010405 organic chemistry Chemistry Topoisomerase DNA 0104 chemical sciences Enzyme Activation Enzyme biology.protein Growth inhibition DNA Topoisomerases |
| Zdroj: | Journal of Inorganic Biochemistry. 168:1-12 |
| ISSN: | 0162-0134 |
| Popis: | This study introduces a pair of newly synthesized silver complexes, [Ag2(HGly)2]n(NO3)2n (1) and [Ag(Nam)2]NO3·H2O (2) (Gly – glycine, Nam – nicotinamide), that were prepared and characterized by relevant methods in solid state (elemental, spectral, thermal and structural analysis) and their stability in solution was verified by 1H NMR measurements. Moreover, suitable reaction conditions were observed by potentiometry depending on pH in case of binary system Ag-Gly. X-ray analysis confirmed argentophilic interactions in complex 1 with an Ag1-Ag2 distance of 2.8018(6) A. Antimicrobial testing indicates higher growth inhibition effect of complex 1 than complex 2. Moreover the effectivity of both complexes against bacteria (Staphylococcus aureus and Escherichia coli) is superior (or similar) to that of the commercially available Ag(I) sulfadiazine, AgSD (used, for example, in Dermazine cream). The binding of the Ag(I) complexes to calf thymus DNA was investigated using electronic absorption, fluorescence and circular dichroism spectrophotometry. The Stern–Volmer quenching constants obtained from the linear quenching plot were estimated in the range from 2.01 × 103 to 20.34 × 103 M− 1. The results of topoisomerase I and topoisomerase II (Topo I and Topo II) inhibition assay suggested that complex 2 inhibits the enzyme activity of both enzymes at a concentration of 2 μM. The cytotoxicity of both complexes on L1210 leukemia cells was revealed to be approximately three times higher than that of cisplatin. Moreover, the new Ag(I) complexes also induced apoptosis of the leukemia cells. The high DNA binding activity of these complexes is considered to be responsible for their cytotoxic effects. |
| Databáze: | OpenAIRE |
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