Biomimetic carriers mimicking leukocyte plasma membrane to increase tumor vasculature permeability
| Autor: | Roberto Molinaro, Franco Salvatore, Mauro Ferrari, Shilpa Scaria, Junping You, Claudia Corbo, Michael Evangelopoulos, Iman K. Yazdi, Ennio Tasciotti, Stefania Acciardo, E. De Rosa, Roberto Palomba, N. E. Toledano Furman, Alessandro Parodi |
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| Přispěvatelé: | Palomba, R, Parodi, A, Evangelopoulos, M, Acciardo, S, Corbo, C, De Rosa, E, Yazdi, I, Scaria, S, Molinaro, R, Furman, N, You, J, Ferrari, M, Salvatore, F, Tasciotti, E |
| Rok vydání: | 2015 |
| Předmět: |
0301 basic medicine
Silicon Endothelium Intercellular Adhesion Molecule-1 Vascular permeability 02 engineering and technology Article Mice 03 medical and health sciences Jurkat Cells Nanopores Immune system Drug Delivery Systems Biomimetic Materials Neoplasms medicine Human Umbilical Vein Endothelial Cells Leukocytes Animals Humans tumors vasculature permeability drug delivery biomimicry Cell Membrane Female Mice Inbred BALB C Neovascularization Pathologic Inbred BALB C Neovascularization Barrier function Pathologic Tumor microenvironment Multidisciplinary Chemistry 021001 nanoscience & nanotechnology Cell biology Surface coating 030104 developmental biology medicine.anatomical_structure Membrane protein 0210 nano-technology |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Recent advances in the field of nanomedicine have demonstrated that biomimicry can further improve targeting properties of current nanotechnologies while simultaneously enable carriers with a biological identity to better interact with the biological environment. Immune cells for example employ membrane proteins to target inflamed vasculature, locally increase vascular permeability and extravasate across inflamed endothelium. Inspired by the physiology of immune cells, we recently developed a procedure to transfer leukocyte membranes onto nanoporous silicon particles (NPS), yielding Leukolike Vectors (LLV). LLV are composed of a surface coating containing multiple receptors that are critical in the cross-talk with the endothelium, mediating cellular accumulation in the tumor microenvironment while decreasing vascular barrier function. We previously demonstrated that lymphocyte function-associated antigen (LFA-1) transferred onto LLV was able to trigger the clustering of intercellular adhesion molecule 1 (ICAM-1) on endothelial cells. Herein, we provide a more comprehensive analysis of the working mechanism of LLV in vitro in activating this pathway and in vivo in enhancing vascular permeability. Our results suggest the biological activity of the leukocyte membrane can be retained upon transplant onto NPS and is critical in providing the particles with complex biological functions towards tumor vasculature. |
| Databáze: | OpenAIRE |
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