Effects of L-Theanine on Posttraumatic Stress Disorder Induced Changes in Rat Brain Gene Expression
| Autor: | William Luke Townsend, Jessica Wojcicki, Joshua Luke Kemper, Gina Padron, Stephanie Martinson, Geno M. Herron, Jason Washington, Tomás Eduardo Ceremuga, Damali Crawford, Robert Edwards, George A. Ceremuga, Robert Revels, Michael Bentley |
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| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Male
medicine.medical_specialty Pathology Article Subject Midazolam Hippocampus lcsh:Medicine behavioral disciplines and activities Amygdala lcsh:Technology General Biochemistry Genetics and Molecular Biology Beverages Rats Sprague-Dawley Stress Disorders Post-Traumatic Glutamates Internal medicine Gene expression mental disorders medicine Animals lcsh:Science General Environmental Science Regulation of gene expression Analysis of Variance Substance dependence business.industry lcsh:T Reverse Transcriptase Polymerase Chain Reaction lcsh:R Brain General Medicine medicine.disease Rats Endocrinology medicine.anatomical_structure Mood disorders Gene Expression Regulation Anxiety lcsh:Q Analysis of variance medicine.symptom business Research Article |
| Zdroj: | The Scientific World Journal The Scientific World Journal, Vol 2014 (2014) |
| ISSN: | 1537-744X 2356-6140 |
| Popis: | Posttraumatic stress disorder (PTSD) is characterized by the occurrence of a traumatic event that is beyond the normal range of human experience. The future of PTSD treatment may specifically target the molecular mechanisms of PTSD. In the US, approximately 20% of adults report taking herbal products to treat medical illnesses. L-theanine is the amino acid in green tea primarily responsible for relaxation effects. No studies have evaluated the potential therapeutic properties of herbal medications on gene expression in PTSD. We evaluated gene expression in PTSD-induced changes in the amygdala and hippocampus of Sprague-Dawley rats. The rats were assigned to PTSD-stressed and nonstressed groups that received either saline, midazolam, L-theanine, or L-theanine + midazolam. Amygdala and hippocampus tissue samples were analyzed for changes in gene expression. One-way ANOVA was used to detect significant difference between groups in the amygdala and hippocampus. Of 88 genes examined, 17 had a large effect size greater than 0.138. Of these, 3 genes in the hippocampus and 5 genes in the amygdala were considered significant (P<0.05) between the groups. RT-PCR analysis revealed significant changes between groups in several genes implicated in a variety of disorders ranging from PTSD, anxiety, mood disorders, and substance dependence. |
| Databáze: | OpenAIRE |
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