Study on toxin-neutralization properties of recombinant single-chain variable antibody's fragments against diphtheria toxin B subunit
| Autor: | S. V. Komisarenko, Kolibo Dv, N. V. Korotkevich, O. S. Oliinyk, A. J. Labyntsev |
|---|---|
| Předmět: |
Diphtheria toxin
biology QH301-705.5 Toxin Chemistry Protein subunit antitoxins chemical and pharmacologic phenomena Single chain respiratory system QH426-470 medicine.disease_cause Molecular biology scFv General Biochemistry Genetics and Molecular Biology Neutralization law.invention law diphtheria toxin Genetics biology.protein medicine Recombinant DNA Biology (General) Antibody |
| Zdroj: | Scopus-Elsevier Biopolymers and Cell, Vol 25, Iss 4, Pp 315-318 (2009) |
| Popis: | Aim. To select scFv which have an ability to neutralize diphtheria toxin. Methods. Flow-cytometry was used to study the ability of specific scFv to block the binding of a labeled receptor binding fragment of toxin with a surface of sensitive to toxin cells. Results. Four scFv with toxin-neutralization properties were selected. Conclusions. The obtained scFv could be considered as promising candidates for antitoxic antidiphtheria drugs of new generation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|