A Wiring of the Human Nucleolus
| Autor: | Jens S. Andersen, Søren Brunak, Lars Kiemer, Matthias Mann, Agnieszka S. Juncker, E. Olof Karlberg, Kasper Lage, Anders M. Hinsby, Anders Fausbøll |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Proteomics
Databases Factual Proteome Nucleolus Genetic Variation Reproducibility of Results Ribosome biogenesis Cell Biology Biology Models Biological Cell biology Artificial Intelligence Software Design Nucleolar Proteins Organelle Humans Identification (biology) Ribosomes Molecular Biology Cell Nucleolus Function (biology) |
| Zdroj: | Hinsby, A M, Kiemer, L, Karlberg, E O, Lage, K, Fausbøll, A, Juncker, A S, Andersen, J S, Mann, M & Brunak, S 2006, ' A wiring of the human nucleolus ', Molecular Cell, vol. 22, pp. 285-295 . University of Southern Denmark |
| ISSN: | 1097-2765 |
| DOI: | 10.1016/j.molcel.2006.03.012 |
| Popis: | Recent proteomic efforts have created an extensive inventory of the human nucleolar proteome. However, approximately 30% of the identified proteins lack functional annotation. We present an approach of assigning function to uncharacterized nucleolar proteins by data integration coupled to a machine-learning method. By assembling protein complexes, we present a first draft of the human ribosome biogenesis pathway encompassing 74 proteins and hereby assign function to 49 previously uncharacterized proteins. Moreover, the functional diversity of the nucleolus is underlined by the identification of a number of protein complexes with functions beyond ribosome biogenesis. Finally, we were able to obtain experimental evidence of nucleolar localization of 11 proteins, which were predicted by our platform to be associates of nucleolar complexes. We believe other biological organelles or systems could be "wired" in a similar fashion, integrating different types of data with high-throughput proteomics, followed by a detailed biological analysis and experimental validation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|