Genes expressed in Brugia malayi infective third stage larvae
| Autor: | Steven A. Williams, Mark Blaxter, Wenhong Lu, Barton E. Slatko, Alan L. Scott, Inca Ghosh, David B. Guiliano, Nithyakalyani Raghavan |
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| Rok vydání: | 1996 |
| Předmět: |
DNA
Complementary Molecular Sequence Data Protein Sorting Signals Polymerase Chain Reaction Brugia malayi law.invention Elephantiasis Filarial law Sequence Homology Nucleic Acid Complementary DNA parasitic diseases Gene expression Animals Humans Amino Acid Sequence Caenorhabditis elegans Molecular Biology Gene Genes Helminth Polymerase chain reaction DNA Primers Gene Library Genetics Expressed sequence tag Base Sequence Sequence Homology Amino Acid biology cDNA library Gene Expression Regulation Developmental biology.organism_classification Molecular biology Introns Reverse transcriptase Rats Larva Macaca Parasitology |
| Zdroj: | Molecular and Biochemical Parasitology. 77:77-93 |
| ISSN: | 0166-6851 |
| DOI: | 10.1016/0166-6851(96)02571-6 |
| Popis: | We have used a tag sequencing approach to survey genes expressed in the third stage infective larvae of the human filarial nematode parasite Brugia malayi. RNA was isolated from late vector-stage L3 larvae after days 9 or 10 of infection in mosquitos, and converted to cDNA by reverse transcriptase. Double-stranded cDNA was produced by either conventional methods (non-SL cDNA library) or by PCR using the nematode spliced leader (SLI) and oligo(dT) primers (SL cDNA library). Two clone libraries (one from SL and one from non-SL cDNAs) were constructed in lambda ZapII. A set of these full-length clones was selected and 596 inserts were sequenced from the 5' end. We have identified 364 B. malayi genes (the majority of which are new) that encode housekeeping proteins, structural proteins, proteins of immediate immunological or drug-discovery interest as well as a large class of novel sequences which may prove to have significant involvement in host invasion. Extensive, genome-wide approaches to the analysis of larval gene expression are now possible for B. malayi. We present several examples of this approach. |
| Databáze: | OpenAIRE |
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