The maturation of murine dendritic cells induced by human adenovirus is mediated by the fiber knob domain
| Autor: | Pierre Boulanger, Valérie Molinier-Frenkel, Jean-Gérard Guillet, Armelle Prévost-Blondel, Saw-See Hong, Sarah Boudaly, Renée Lengagne, Maria K. Magnusson |
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| Přispěvatelé: | [Institut Cochin] Departement Infection, immunité, inflammation, Institut Cochin (IC UM3 (UMR 8104 / U1016)), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Got-A-Gene AB, Stena Center 1B, Virologie et pathogenèse virale (VPV), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Pathologie métabolique et hormonale du développement, Institut National de la Santé et de la Recherche Médicale (INSERM), We thank Profs. H. Pircher and R. Fortuner for critical reading of the English manuscript, Dr. M. Lusky and Dr. H. Kroenenberger for fruitful discussions, L. Franqueville for help with fast protein liquid chromatography purification of viral proteins, and M. Plut for animal care., Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Cochin ( UM3 (UMR 8104 / U1016) ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Virologie et pathogenèse virale ( VPV ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique ( CNRS ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) |
| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
T-Cell/physiology
Adenoviruses viruses Mutant CD8-Positive T-Lymphocytes Inbred C57BL Biochemistry Membrane Glycoproteins/physiology law.invention Mice 0302 clinical medicine law Receptors Fiber Dendritic Cells/*physiology Receptor Non-U.S. Gov't OCIS 000.1430 Capsid Proteins/*chemistry/*physiology Cells Cultured 0303 health sciences Membrane Glycoproteins Cultured Toll-Like Receptors 3. Good health Capsid 030220 oncology & carcinogenesis Antigen [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology Recombinant DNA Cell Surface/physiology Cells Receptors Antigen T-Cell Receptors Cell Surface Biology CD8-Positive T-Lymphocytes/immunology Research Support [ SDV.MP.VIR ] Life Sciences [q-bio]/Microbiology and Parasitology/Virology 03 medical and health sciences Animals Molecular Biology 030304 developmental biology Adenoviruses Human Capsomere Human/*physiology Dendritic Cells Cell Biology Molecular biology Mice Inbred C57BL Capsid Proteins Immunization CD8 |
| Popis: | We investigated the mechanism of adenovirus serotype 5 (Ad5)-mediated maturation of bone marrow-derived murine dendritic cells (DC) using (i) Ad5 vectors with wild-type capsid (AdE1 degrees, AdGFP); (ii) Ad5 vector mutant deleted of the fiber C-terminal knob domain (AdGFPDeltaknob); and (iii) capsid components isolated from Ad5-infected cells or expressed as recombinant proteins, hexon, penton, penton base, full-length fiber, fiber knob, and fiber mutants. We found that penton capsomer (penton base linked to its fiber projection), full-length fiber protein, and its isolated knob domain were all capable of inducing DC maturation, whereas no significant DC maturation was observed for hexon or penton base alone. This capacity was severely reduced for AdGFPDeltaknob and for fiber protein deletion mutants lacking the beta-stranded region F of the knob (residues Leu-485-Thr-486). The DC maturation effect was fully retained in a recombinant fiber protein deleted of the HI loop (FiDeltaHI), a fiber (Fi) deletion mutant that failed to trimerize, suggesting that the fiber knob-mediated DC activation did not depend on the integrity of the HI loop and on the trimeric status of the fiber. Interestingly, peptide-pulsed DC that had been stimulated with Ad5 knob protein induced a potent CD8+ T cell response in vivo. |
| Databáze: | OpenAIRE |
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