Amelioration of airway inflammation and pulmonary edema by Teucrium stocksianum via attenuation of pro-inflammatory cytokines and up-regulation of AQP1 and AQP5
| Autor: | Muhammad Irfan Basheer, Nawazish-i-Husain Syed, Marriam Sharif, Basma Ijaz, Muhammad Shahzad, Aisha Mobashar, Arham Shabbir, Rasool Baksh Tareen |
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| Rok vydání: | 2020 |
| Předmět: |
Pulmonary and Respiratory Medicine
Male Physiology Respiratory Tract Diseases Pulmonary Edema Pharmacology Proinflammatory cytokine Teucrium 03 medical and health sciences Mice 0302 clinical medicine medicine Hypersensitivity Animals Immunologic Factors Interleukin 5 Interleukin 4 Inflammation Mice Inbred BALB C Lung biology Aquaporin 1 Chemistry Plant Extracts General Neuroscience respiratory system Pulmonary edema medicine.disease Asthma Aquaporin 5 Reverse transcription polymerase chain reaction Ovalbumin Disease Models Animal medicine.anatomical_structure 030228 respiratory system biology.protein Nasal administration Female Interleukin-4 Interleukin-5 030217 neurology & neurosurgery |
| Zdroj: | Respiratory physiologyneurobiology. 284 |
| ISSN: | 1878-1519 |
| Popis: | Current study investigates the immunomodulatory effects of T. stocksianum using mouse model of ovalbumin (OVA)-induced allergic asthma. The mice were treated with methanolic extract, n-hexane, and ethyl acetate fractions for consecutive 7 days along with intranasal challenge. The mRNA expression levels of interleukin-4 (IL-4), IL-5, Aquaporin-1 (AQP1) and Aquaporin-5 (AQP5) were evaluated using reverse transcription polymerase chain reaction. The data showed that T. stocksianum significantly reduced airway inflammation as indicated by reduced inflammatory cell infiltration in lungs, and attenuated total and differential leukocyte counts both in blood and BALF. Expression levels of pro-inflammatory IL-4 and IL-5 in lungs were also found significantly reduced. T. stocksianum significantly reduced pulmonary edema as indicated by reduced lung wet/dry ratio and goblet cell hyperplasia. AQP1 and AQP5 expression levels were also found elevated in treatment groups. In conclusion, T. stocksianum possesses anti-asthmatic activity which may be attributed to reduction in IL-4 and IL-5 expression levels, and elevation in AQP1 and AQP5 expression levels. |
| Databáze: | OpenAIRE |
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