Effects of lamellolysin from a parasitoid wasp onDrosophila blood cells in vitro
| Autor: | Rose M. Rizki, T. M. Rizki |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Male
Hemocytes Wasps Parasitoid wasp Parasitoid Biological Factors Drosophilidae Hemolymph Botany Cell Adhesion Melanogaster Animals Cells Cultured Crosses Genetic biology fungi General Medicine biology.organism_classification In vitro Cell biology Drosophila melanogaster Larva Mutation Female Animal Science and Zoology Leptopilina heterotoma |
| Zdroj: | Journal of Experimental Zoology. 257:236-244 |
| ISSN: | 1097-010X 0022-104X |
| DOI: | 10.1002/jez.1402570214 |
| Popis: | Female parasitoid Leptopilina heterotoma inject a factor, lamellolysin, along with their eggs into the host hemocoel to destroy selectively host hemocytes that encapsulate foreign objects. In parasitized Drosophila melanogaster larvae, these hemocytes (lamellocytes) change from discoidal cells to bipolar cells that no longer adhere to each other to form capsules. To study the effects of lamellolysin on Drosophila lamellocytes in vitro, a giant strain of D. melanogaster was constructed to yield hemolymph with an abundance of lamellocytes. The effect of lamellolysin on the adhesivity of lamellocytes in vitro was demonstrated when the cells were gently rotated in the culture medium. Under these conditions, the bipolar shape of the affected lamellocytes resembled that of lamellocytes in parasitized hosts. When lamellocytes were exposed to lamellolysin in stationary culture medium, the elongation of the bipolar cells continued until they became threadlike. Lamellocytes fragmented in both stationary and rotating culture medium in the presence of lamellolysin, although loss of cellular material was more pronounced in the latter. This study demonstrates that lamellolysin acts directly and destructively on lamellocytes. |
| Databáze: | OpenAIRE |
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